Enhanced bioremediation of n-alkane in petroleum sludge using bacterial consortium amended with rhamnolipid and micronutrients 

Hdl Handle:
http://hdl.handle.net/10149/58339
Title:
Enhanced bioremediation of n-alkane in petroleum sludge using bacterial consortium amended with rhamnolipid and micronutrients 
Authors:
Rahman, P. K. S. M. (Pattanathu); Rahman, T. J. (Thahira); Kourkoutas, Y. (Yiannis); Petsas, I.; Marchant, R. (Roger); Banat, I. M. (Ibrahim)
Affiliation:
University of Ulster. School of Biological and Environmental Sciences. Biotechnology Research Group.
Citation:
Rahman, K. S. M. et al. (2003) 'Enhanced bioremediation of n-alkane in petroleum sludge using bacterial consortium amended with rhamnolipid and micronutrients', Bioresource Technology, 90 (2), pp.159-168.
Publisher:
Elsevier
Journal:
Bioresource Technology
Issue Date:
Nov-2003
URI:
http://hdl.handle.net/10149/58339
DOI:
10.1016/S0960-8524(03)00114-7
Abstract:
The purpose of the present study was to investigate possible methods to enhance the rate of biodegradation of oil sludge from crude oil tank bottom, thus reducing the time usually required for bioremediation. Enhancement of biodegradation was achieved through bioaugmentation and biostimulation. About 10% and 20% sludge contaminated sterile and non-sterile soil samples were treated with bacterial consortium (BC), rhamnolipid biosurfactant (RL) and nitrogen, phosphorus and potassium (NPK) solution. Maximum n-alkane degradation occurred in the 10% sludge contaminated soil samples. The effects of treatment carried out with the non-sterile soil samples were more pronounced than in the sterile soils. Maximum degradation was achieved after the 56th day of treatment. n-Alkanes in the range of nC8–nC11 were degraded completely followed by nC12–nC21, nC22–nC31 and nC32–nC40 with percentage degradations of 100%, 83–98%, 80–85% and 57–73% respectively. Statistical analysis using analysis of variance and Duncan’s multiple range test revealed that the level of amendments, incubation time and combination of amendments significantly influenced bacterial growth, protein concentration and surface tension at a 1% probability level. All tested additives BC, NPK and RL had significant positive effects on the bioremediation of n-alkane in petroleum sludge.
Type:
Article
Keywords:
tank bottom sludge; bacterial consortium; rhamnolipid; bioremediation; bioaugmentation
ISSN:
0960-8524
Rights:
Author can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 04/01/2010]
Citation Count:
56 [Scopus, 04/01/2010]

Full metadata record

DC FieldValue Language
dc.contributor.authorRahman, P. K. S. M. (Pattanathu)-
dc.contributor.authorRahman, T. J. (Thahira)-
dc.contributor.authorKourkoutas, Y. (Yiannis)-
dc.contributor.authorPetsas, I.-
dc.contributor.authorMarchant, R. (Roger)-
dc.contributor.authorBanat, I. M. (Ibrahim)-
dc.date.accessioned2009-04-01T10:49:29Z-
dc.date.available2009-04-01T10:49:29Z-
dc.date.issued2003-11-
dc.identifier.citationBioresource Technology; 90 (2): 159-168-
dc.identifier.issn0960-8524-
dc.identifier.doi10.1016/S0960-8524(03)00114-7-
dc.identifier.urihttp://hdl.handle.net/10149/58339-
dc.description.abstractThe purpose of the present study was to investigate possible methods to enhance the rate of biodegradation of oil sludge from crude oil tank bottom, thus reducing the time usually required for bioremediation. Enhancement of biodegradation was achieved through bioaugmentation and biostimulation. About 10% and 20% sludge contaminated sterile and non-sterile soil samples were treated with bacterial consortium (BC), rhamnolipid biosurfactant (RL) and nitrogen, phosphorus and potassium (NPK) solution. Maximum n-alkane degradation occurred in the 10% sludge contaminated soil samples. The effects of treatment carried out with the non-sterile soil samples were more pronounced than in the sterile soils. Maximum degradation was achieved after the 56th day of treatment. n-Alkanes in the range of nC8–nC11 were degraded completely followed by nC12–nC21, nC22–nC31 and nC32–nC40 with percentage degradations of 100%, 83–98%, 80–85% and 57–73% respectively. Statistical analysis using analysis of variance and Duncan’s multiple range test revealed that the level of amendments, incubation time and combination of amendments significantly influenced bacterial growth, protein concentration and surface tension at a 1% probability level. All tested additives BC, NPK and RL had significant positive effects on the bioremediation of n-alkane in petroleum sludge.-
dc.publisherElsevier-
dc.rightsAuthor can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 04/01/2010]-
dc.subjecttank bottom sludge-
dc.subjectbacterial consortium-
dc.subjectrhamnolipid-
dc.subjectbioremediation-
dc.subjectbioaugmentation-
dc.titleEnhanced bioremediation of n-alkane in petroleum sludge using bacterial consortium amended with rhamnolipid and micronutrients -
dc.typeArticle-
dc.contributor.departmentUniversity of Ulster. School of Biological and Environmental Sciences. Biotechnology Research Group.-
dc.identifier.journalBioresource Technology-
ref.assessmentRAE 2008-
ref.citationcount56 [Scopus, 04/01/2010]-
or.citation.harvardRahman, K. S. M. et al. (2003) 'Enhanced bioremediation of n-alkane in petroleum sludge using bacterial consortium amended with rhamnolipid and micronutrients', Bioresource Technology, 90 (2), pp.159-168.-
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