Hdl Handle:
http://hdl.handle.net/10149/58384
Title:
Simple dip strip ELISA for airborne estrogenic steroids
Authors:
Armstrong, S. (Stewart); Miao, Z. F. (Zhi Feng); Rowell, F. J. (Frederick); Ali, Z. (Zulfiqur)
Affiliation:
University of Sunderland. School of Sciences. Institute of Pharmacy and Chemistry. North East Biotechnology Centre; University of Teesside. School of Science and Technology. Biosensors Group.
Citation:
Armstrong, S. et al. (2001) 'Simple dip strip ELISA for airborne estrogenic steroids', Analytica Chimica Acta, 444 (1), pp.79-86.
Publisher:
Elsevier
Journal:
Analytica Chimica Acta
Issue Date:
Oct-2001
URI:
http://hdl.handle.net/10149/58384
DOI:
10.1016/S0003-2670(01)01165-5
Abstract:
An ELISA for the potent estrogenic steroids, ethinyl estradiol (ETED), 17-β-estradiol (ED) and estrone (ES) has been developed and used to determine the recovery of ED and ETED following spiking from seven filters commonly used in samplers for ascertaining personal exposure of workers to airborne biochemicals. Best results were obtained with cellulose nitrate (CN) and polytetrafluoroethylene (PTFE) filters. The assay reagents have also been used to develop a simple dip strip assay that can be used to determine the presence of steroids on the filters. Steroids captured from the air on the surface of filters within a conventional personal exposure sampler are extracted with specific antiserum. A spot of hapten-protein conjugate is immobilised on a small square of CN filter attached to a plastic strip. This is immersed in the sampler solution where unbound antibodies bind to the hapten on the spot. The strips are then transferred to a 96 well filter plate located within a filter manifold. Following washing, strips are incubated with alkaline phosphatase-labelled second antibody and the spots were developed by addition of substrate. The intensity of the developed blue spot is inversely proportional to the amount of steroid originally captured on the filter. A batch of over 50 samplers can be screened within 1 h. Spots on strips from filters on which 10 ng of ED, ES or ETED are present can be visually discerned from strips from filters where no steroid is present.
Type:
Article
Keywords:
airborne estrogenic steroids; health and safety; recovery from filters; dip strip ELISA
ISSN:
0003-2670
Rights:
Author can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 21/12/09]
Citation Count:
8 [Scopus, 21/12/09]

Full metadata record

DC FieldValue Language
dc.contributor.authorArmstrong, S. (Stewart)-
dc.contributor.authorMiao, Z. F. (Zhi Feng)-
dc.contributor.authorRowell, F. J. (Frederick)-
dc.contributor.authorAli, Z. (Zulfiqur)-
dc.date.accessioned2009-04-01T10:50:40Z-
dc.date.available2009-04-01T10:50:40Z-
dc.date.issued2001-10-
dc.identifier.citationAnalytica Chimica Acta; 444 (1): 79-86-
dc.identifier.issn0003-2670-
dc.identifier.doi10.1016/S0003-2670(01)01165-5-
dc.identifier.urihttp://hdl.handle.net/10149/58384-
dc.description.abstractAn ELISA for the potent estrogenic steroids, ethinyl estradiol (ETED), 17-β-estradiol (ED) and estrone (ES) has been developed and used to determine the recovery of ED and ETED following spiking from seven filters commonly used in samplers for ascertaining personal exposure of workers to airborne biochemicals. Best results were obtained with cellulose nitrate (CN) and polytetrafluoroethylene (PTFE) filters. The assay reagents have also been used to develop a simple dip strip assay that can be used to determine the presence of steroids on the filters. Steroids captured from the air on the surface of filters within a conventional personal exposure sampler are extracted with specific antiserum. A spot of hapten-protein conjugate is immobilised on a small square of CN filter attached to a plastic strip. This is immersed in the sampler solution where unbound antibodies bind to the hapten on the spot. The strips are then transferred to a 96 well filter plate located within a filter manifold. Following washing, strips are incubated with alkaline phosphatase-labelled second antibody and the spots were developed by addition of substrate. The intensity of the developed blue spot is inversely proportional to the amount of steroid originally captured on the filter. A batch of over 50 samplers can be screened within 1 h. Spots on strips from filters on which 10 ng of ED, ES or ETED are present can be visually discerned from strips from filters where no steroid is present.-
dc.publisherElsevier-
dc.rightsAuthor can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 21/12/09]-
dc.subjectairborne estrogenic steroids-
dc.subjecthealth and safety-
dc.subjectrecovery from filters-
dc.subjectdip strip ELISA-
dc.titleSimple dip strip ELISA for airborne estrogenic steroids-
dc.typeArticle-
dc.contributor.departmentUniversity of Sunderland. School of Sciences. Institute of Pharmacy and Chemistry. North East Biotechnology Centre; University of Teesside. School of Science and Technology. Biosensors Group.-
dc.identifier.journalAnalytica Chimica Acta-
ref.assessmentRAE 2008-
ref.citationcount8 [Scopus, 21/12/09]-
or.citation.harvardArmstrong, S. et al. (2001) 'Simple dip strip ELISA for airborne estrogenic steroids', Analytica Chimica Acta, 444 (1), pp.79-86.-
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