Longitudinal development of the gut microbiome and metabolome in preterm neonates with late onset sepsis and healthy controls

Hdl Handle:
http://hdl.handle.net/10149/621294
Title:
Longitudinal development of the gut microbiome and metabolome in preterm neonates with late onset sepsis and healthy controls
Authors:
Stewart, Christopher J.; Embleton, Nicholas D.; Marrs, Emma C. L.; Smith, Daniel P.; Fofanova, Tatiana; Nelson, Andrew; Skeath, Tom; Perry, John D.; Petrosino, Joseph F.; Berrington, Janet E.; Cummings, S. P. (Stephen) ( 0000-0001-5298-2075 )
Affiliation:
Teesside University. Technology Futures Institute
Citation:
Stewart CJ, Embleton ND, Marrs ECL, Smith DP, Fofanova T, Nelson A, Skeath T, Perry JD, Petrosino JF, Berrington JE, Cummings SP. (2017) 'Longitudinal development of the gut microbiome and metabolome in preterm neonates with late onset sepsis and healthy controls' Microbiome 5 (1)
Publisher:
BioMed Central
Journal:
Microbiome
Issue Date:
12-Jul-2017
URI:
http://hdl.handle.net/10149/621294
DOI:
10.1186/s40168-017-0295-1
Additional Links:
http://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-017-0295-1
Abstract:
Background Late onset sepsis (LOS) in preterm infants is associated with considerable morbidity and mortality. While studies have implicated gut bacteria in the aetiology of the disease, functional analysis and mechanistic insights are generally lacking. We performed temporal bacterial (n = 613) and metabolomic (n = 63) profiling on extensively sampled stool from 7 infants with LOS and 28 matched healthy (no LOS or NEC) controls. Results The bacteria isolated in diagnostic blood culture usually corresponded to the dominant bacterial genera in the gut microbiome. Longitudinal changes were monitored based on preterm gut community types (PGCTs), where control infants had an increased number of PGCTs compared to LOS infants (P = 0.011). PGCT 6, characterised by Bifidobacteria dominance, was only present in control infants. Metabolite profiles differed between LOS and control infants at diagnosis and 7 days later, but not 7 days prior to diagnosis. Bifidobacteria was positively correlated with control metabolites, including raffinose, sucrose, and acetic acid. Conclusions Using multi-omic analysis, we show that the gut microbiome is involved in the pathogenesis of LOS. While the causative agent of LOS varies, it is usually abundant in the gut. Bifidobacteria dominance was associated with control infants, and the presence of this organism may directly protect, or act as a marker for protection, against gut epithelial translocation. While the metabolomic data is preliminary, the findings support that gut development and protection in preterm infants is associated with increased in prebiotic oligosaccharides (e.g. raffinose) and the growth of beneficial bacteria (e.g. Bifidobacterium).
Type:
Article
Language:
en
ISSN:
2049-2618
Rights:
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/)

Full metadata record

DC FieldValue Language
dc.contributor.authorStewart, Christopher J.en
dc.contributor.authorEmbleton, Nicholas D.en
dc.contributor.authorMarrs, Emma C. L.en
dc.contributor.authorSmith, Daniel P.en
dc.contributor.authorFofanova, Tatianaen
dc.contributor.authorNelson, Andrewen
dc.contributor.authorSkeath, Tomen
dc.contributor.authorPerry, John D.en
dc.contributor.authorPetrosino, Joseph F.en
dc.contributor.authorBerrington, Janet E.en
dc.contributor.authorCummings, S. P. (Stephen)en
dc.date.accessioned2017-07-17T14:18:15Z-
dc.date.available2017-07-17T14:18:15Z-
dc.date.issued2017-07-12-
dc.identifier.issn2049-2618-
dc.identifier.doi10.1186/s40168-017-0295-1-
dc.identifier.urihttp://hdl.handle.net/10149/621294-
dc.description.abstractBackground Late onset sepsis (LOS) in preterm infants is associated with considerable morbidity and mortality. While studies have implicated gut bacteria in the aetiology of the disease, functional analysis and mechanistic insights are generally lacking. We performed temporal bacterial (n = 613) and metabolomic (n = 63) profiling on extensively sampled stool from 7 infants with LOS and 28 matched healthy (no LOS or NEC) controls. Results The bacteria isolated in diagnostic blood culture usually corresponded to the dominant bacterial genera in the gut microbiome. Longitudinal changes were monitored based on preterm gut community types (PGCTs), where control infants had an increased number of PGCTs compared to LOS infants (P = 0.011). PGCT 6, characterised by Bifidobacteria dominance, was only present in control infants. Metabolite profiles differed between LOS and control infants at diagnosis and 7 days later, but not 7 days prior to diagnosis. Bifidobacteria was positively correlated with control metabolites, including raffinose, sucrose, and acetic acid. Conclusions Using multi-omic analysis, we show that the gut microbiome is involved in the pathogenesis of LOS. While the causative agent of LOS varies, it is usually abundant in the gut. Bifidobacteria dominance was associated with control infants, and the presence of this organism may directly protect, or act as a marker for protection, against gut epithelial translocation. While the metabolomic data is preliminary, the findings support that gut development and protection in preterm infants is associated with increased in prebiotic oligosaccharides (e.g. raffinose) and the growth of beneficial bacteria (e.g. Bifidobacterium).en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-017-0295-1en
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/)en
dc.titleLongitudinal development of the gut microbiome and metabolome in preterm neonates with late onset sepsis and healthy controlsen
dc.typeArticleen
dc.contributor.departmentTeesside University. Technology Futures Instituteen
dc.identifier.journalMicrobiomeen
or.citation.harvardStewart CJ, Embleton ND, Marrs ECL, Smith DP, Fofanova T, Nelson A, Skeath T, Perry JD, Petrosino JF, Berrington JE, Cummings SP. (2017) 'Longitudinal development of the gut microbiome and metabolome in preterm neonates with late onset sepsis and healthy controls' Microbiome 5 (1)en
dc.eprint.versionPublisher's Version/PDFen
dc.embargoNoneen
dc.date.accepted2017-06-29-
All Items in TeesRep are protected by copyright, with all rights reserved, unless otherwise indicated.