Design, optimisation and preliminary validation of a human specific loop-mediated amplification assay for the rapid detection of human DNA at forensic crime scenes

Hdl Handle:
http://hdl.handle.net/10149/621382
Title:
Design, optimisation and preliminary validation of a human specific loop-mediated amplification assay for the rapid detection of human DNA at forensic crime scenes
Authors:
Hird, H. J.; Brown, M. K. (Melanie)
Affiliation:
Teesside University. Technology Futures Institute
Citation:
Hird, H. J., Brown, M. K. (2017) 'Design, optimisation and preliminary validation of a human specific loop-mediated amplification assay for the rapid detection of human DNA at forensic crime scenes' Science & Justice; Online first 3 Aug 2017 : DOI: 10.1016/j.scijus.2017.08.001
Publisher:
Elsevier
Journal:
Science & Justice
Issue Date:
3-Aug-2017
URI:
http://hdl.handle.net/10149/621382
DOI:
10.1016/j.scijus.2017.08.001
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S1355030617300904
Abstract:
The identification of samples at a crime scene which require forensic DNA typing has been the focus of recent research interest. We propose a simple, but sensitive analysis system which can be deployed at a crime scene to identify crime scene stains as human or non-human. The proposed system uses the isothermal amplification of DNA in a rapid assay format, which returns results in as little as 30 min from sampling. The assay system runs on the Genie II device, a proven in-field detection system which could be deployed at a crime scene. The results presented here demonstrate that the system was sufficiently specific and sensitive and was able to detect the presence of human blood, semen and saliva on mock forensic samples.
Type:
Article
Language:
en
ISSN:
1355-0306
Sponsors:
The authors would like to thank Fera for supporting this project and Optigene for helpful advice. They are also grateful to all the volunteers who donated their samples for this project, to Jenny Tomlinson who provided invaluable advice and to James Chisholm for technical assistance with enumerating the DNA concentration of the MRC-5 cell line and Gaynor Johnson for tissue culture of the MRC-5 cell line.
Rights:
Following a 12 month embargo author can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo/issn/1355-0306/ [Accessed: 11/08/2017]

Full metadata record

DC FieldValue Language
dc.contributor.authorHird, H. J.en
dc.contributor.authorBrown, M. K. (Melanie)en
dc.date.accessioned2017-08-11T15:52:07Z-
dc.date.available2017-08-11T15:52:07Z-
dc.date.issued2017-08-03-
dc.identifier.citationScience & Justice; Online first 3 Aug 2017en
dc.identifier.issn1355-0306-
dc.identifier.doi10.1016/j.scijus.2017.08.001-
dc.identifier.urihttp://hdl.handle.net/10149/621382-
dc.description.abstractThe identification of samples at a crime scene which require forensic DNA typing has been the focus of recent research interest. We propose a simple, but sensitive analysis system which can be deployed at a crime scene to identify crime scene stains as human or non-human. The proposed system uses the isothermal amplification of DNA in a rapid assay format, which returns results in as little as 30 min from sampling. The assay system runs on the Genie II device, a proven in-field detection system which could be deployed at a crime scene. The results presented here demonstrate that the system was sufficiently specific and sensitive and was able to detect the presence of human blood, semen and saliva on mock forensic samples.en
dc.description.sponsorshipThe authors would like to thank Fera for supporting this project and Optigene for helpful advice. They are also grateful to all the volunteers who donated their samples for this project, to Jenny Tomlinson who provided invaluable advice and to James Chisholm for technical assistance with enumerating the DNA concentration of the MRC-5 cell line and Gaynor Johnson for tissue culture of the MRC-5 cell line.en
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S1355030617300904en
dc.rightsFollowing a 12 month embargo author can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo/issn/1355-0306/ [Accessed: 11/08/2017]en
dc.titleDesign, optimisation and preliminary validation of a human specific loop-mediated amplification assay for the rapid detection of human DNA at forensic crime scenesen
dc.typeArticleen
dc.contributor.departmentTeesside University. Technology Futures Instituteen
dc.identifier.journalScience & Justiceen
or.citation.harvardHird, H. J., Brown, M. K. (2017) 'Design, optimisation and preliminary validation of a human specific loop-mediated amplification assay for the rapid detection of human DNA at forensic crime scenes' Science & Justice; Online first 3 Aug 2017 : DOI: 10.1016/j.scijus.2017.08.001-
dc.eprint.versionPost-printen
dc.embargo12 monthsen
dc.date.accepted2017-08-02-
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