Characterization of bacteria in ballast water using MALDI-TOF mass spectrometry

Hdl Handle:
http://hdl.handle.net/10149/296340
Title:
Characterization of bacteria in ballast water using MALDI-TOF mass spectrometry
Authors:
Emami, K. (Kaveh); Askari, V. (Vahid); Ullrich, M (Matthais); Mohinudeen, K. (Khwajah); Anil, A.C. (Arga Chandrashekar); Khandeparker, L. (Lidita); Burgess, J.G. (J. Grant); Mesbahi, E. (Ehsan)
Affiliation:
Teesside University. School of Science and Engineering.
Citation:
Enami, K., Askari, V., Ullrich, M., Mohinudeen, K., Anil, A.C., Khandeparker, L., Burgess, J.G., Mesbahi, E., (2012) 'Characterization of bacteria in ballast water using MALDI-TOF mass spectrometry' PLos ONE 7(6): e38515
Publisher:
PLoS
Journal:
PLoS ONE
Issue Date:
7-Jun-2012
URI:
http://hdl.handle.net/10149/296340
DOI:
10.1371/journal.pone.0038515
Abstract:
To evaluate a rapid and cost-effective method for monitoring bacteria in ballast water, several marine bacterial isolates were characterized by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Since International Maritime Organization (IMO) regulations are concerned with the unintended transportation of pathogenic bacteria through ballast water, emphasis was placed on detecting species of Vibrio, enterococci and coliforms. Seawater samples collected from the North Sea were incubated in steel ballast tanks and the presence of potentially harmful species of Pseudomonas was also investigated. At the genus-level, the identification of thirty six isolates using MALDI-TOF MS produced similar results to those obtained by 16S rRNA gene sequencing. No pathogenic species were detected either by 16S rRNA gene analysis or by MALDI-TOF MS except for the opportunistically pathogenic bacterium Pseudomonas aeruginosa. In addition, in house software that calculated the correlation coefficient values (CCV) of the mass spectral raw data and their variation was developed and used to allow the rapid and efficient identification of marine bacteria in ballast water for the first time.
Type:
Article
Keywords:
vibrio; enterococci; coliforms; marine bacteria; North Sea
Rights:
Author can archive publisher's version/PDF. For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 18/07/2013].
Citation Count:
2 [Web of Science and Scopus, 18/07/2013]

Full metadata record

DC FieldValue Language
dc.contributor.authorEmami, K. (Kaveh)en_GB
dc.contributor.authorAskari, V. (Vahid)en_GB
dc.contributor.authorUllrich, M (Matthais)en_GB
dc.contributor.authorMohinudeen, K. (Khwajah)en_GB
dc.contributor.authorAnil, A.C. (Arga Chandrashekar)en_GB
dc.contributor.authorKhandeparker, L. (Lidita)en_GB
dc.contributor.authorBurgess, J.G. (J. Grant)en_GB
dc.contributor.authorMesbahi, E. (Ehsan)en_GB
dc.date.accessioned2013-07-18T10:47:07Z-
dc.date.available2013-07-18T10:47:07Z-
dc.date.issued2012-06-07-
dc.identifier.citationPLoS ONE; 6 (7) e38515en_GB
dc.identifier.doi10.1371/journal.pone.0038515-
dc.identifier.urihttp://hdl.handle.net/10149/296340-
dc.description.abstractTo evaluate a rapid and cost-effective method for monitoring bacteria in ballast water, several marine bacterial isolates were characterized by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Since International Maritime Organization (IMO) regulations are concerned with the unintended transportation of pathogenic bacteria through ballast water, emphasis was placed on detecting species of Vibrio, enterococci and coliforms. Seawater samples collected from the North Sea were incubated in steel ballast tanks and the presence of potentially harmful species of Pseudomonas was also investigated. At the genus-level, the identification of thirty six isolates using MALDI-TOF MS produced similar results to those obtained by 16S rRNA gene sequencing. No pathogenic species were detected either by 16S rRNA gene analysis or by MALDI-TOF MS except for the opportunistically pathogenic bacterium Pseudomonas aeruginosa. In addition, in house software that calculated the correlation coefficient values (CCV) of the mass spectral raw data and their variation was developed and used to allow the rapid and efficient identification of marine bacteria in ballast water for the first time.en_GB
dc.publisherPLoSen_GB
dc.rightsAuthor can archive publisher's version/PDF. For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 18/07/2013].en_GB
dc.subjectvibrioen_GB
dc.subjectenterococcien_GB
dc.subjectcoliformsen_GB
dc.subjectmarine bacteriaen_GB
dc.subjectNorth Seaen_GB
dc.titleCharacterization of bacteria in ballast water using MALDI-TOF mass spectrometry-
dc.typeArticleen
dc.contributor.departmentTeesside University. School of Science and Engineering.en_GB
dc.identifier.journalPLoS ONEen_GB
ref.citationcount2 [Web of Science and Scopus, 18/07/2013]en_GB
or.citation.harvardEnami, K., Askari, V., Ullrich, M., Mohinudeen, K., Anil, A.C., Khandeparker, L., Burgess, J.G., Mesbahi, E., (2012) 'Characterization of bacteria in ballast water using MALDI-TOF mass spectrometry' PLos ONE 7(6): e38515-
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