Optimisation of biosurfactants produced by non-pathogenic bacteria from environmental samples

Hdl Handle:
http://hdl.handle.net/10149/322898
Title:
Optimisation of biosurfactants produced by non-pathogenic bacteria from environmental samples
Authors:
Uzoigwe, C. I. (Chibuzo); Rahman, P. K. S. M. (Pattanathu); Ennis, C. J. (Chris); Burgess, J. G. (James)
Affiliation:
Teesside University. Technology Futures Institute.
Citation:
Chibuzo I. Uzoigwe, Pattanathu K. S. M. Rahman, Christopher J. Ennis, and James G. Burgess, (2014). Optimisation of biosurfactants produced by non-pathogenic bacteria from environmental samples. Presented at SET for BRITAIN 2014 poster competition at the House of Commons, London on 17th March 2014 organised by Parliamentary and Scientific Committee
Conference:
SET for BRITAIN 2014 poster competition at the House of Commons, organised by Parliamentary and Scientific Committee. London March 17 2014
Issue Date:
Mar-2014
URI:
http://hdl.handle.net/10149/322898
Additional Links:
http://www.setforbritain.org.uk/index.asp; http://www.tees.ac.uk/sections/international/news_story.cfm?story_id=4611&this_issue_title=March%202014&this_issue=250
Abstract:
Biosurfactants refers to surfactants that can be synthesized by several identified microorganisms including bacteria, yeast and fungi. Majority of the surfactants used in the industries today are manufactured from petro-chemical resources and are known as synthetic/chemical surfactants. These compounds are not only partially biodegradable but are also toxic to living organisms and have contributed to a wide range of environmental hazards/health risks. The development of biosurfactants as potential alternatives to synthetic ones, has recently gained world-wide scientific and industrial attention as interesting classes of compounds because of their outstanding characteristics such as; low toxicity, biodegradability, effectiveness at extreme pH, temperature, alkalinity/acidity and ionic strength, effectiveness at low concentration, excellent emulsification/solubilisation activity, can be synthesised by renewable/waste substrates and have the ability to act as antimicrobial as well as anticancer treating agents. However, due to certain technical and economic factors which one of them includes; the pathogenicity of biosurfactant-producing strains, the industrial scale production and application of biosurfactants is still an unachieved task. This research has been designed to identify new/safe non-pathogenic biosurfactant producing bacteria and to improve their rate of biosurfactant production with the aim of introducing them as biological factories and their biosurfactants can be alternative raw materials in the production of household and health care products. A total of 45 bacterial isolates obtained from two oil-contaminated mangrove soil samples collected from Nigeria, have been screened so far for their ability to produce biosurfactants. Screening for biosurfactant production was done in three selected cultivation media; nutrient broth, mineral salt medium+2% glucose and nutrient broth+3% glycerol and positive results were verified by measuring the reduction in surface tension of cell-free culture supernatant using the Du-Noug ring method. Out of the 45 isolates, 12 have turned out as positive biosurfactant producers. The future plans of the research would include; molecular identification of the positive isolates, improving their rate of biosurfactant production using wastes materials like animal fat and glycerol, characterisation of the biosurfactant produced and to determine their cytotoxicity, antimicrobial, anticancer and emulsification properties. The successful identification of safe biosurfactant- producing microbial strains with the ability to produce sufficient amount of surfactant could create a huge opportunity of replacing the synthetic surfactants with the better biosurfactants in all applicable industrial processes.
Type:
Presentation
Language:
en

Full metadata record

DC FieldValue Language
dc.contributor.authorUzoigwe, C. I. (Chibuzo)en
dc.contributor.authorRahman, P. K. S. M. (Pattanathu)en
dc.contributor.authorEnnis, C. J. (Chris)en
dc.contributor.authorBurgess, J. G. (James)en
dc.date.accessioned2014-07-15T10:39:13Zen
dc.date.available2014-07-15T10:39:13Zen
dc.date.issued2014-03en
dc.identifier.urihttp://hdl.handle.net/10149/322898en
dc.description.abstractBiosurfactants refers to surfactants that can be synthesized by several identified microorganisms including bacteria, yeast and fungi. Majority of the surfactants used in the industries today are manufactured from petro-chemical resources and are known as synthetic/chemical surfactants. These compounds are not only partially biodegradable but are also toxic to living organisms and have contributed to a wide range of environmental hazards/health risks. The development of biosurfactants as potential alternatives to synthetic ones, has recently gained world-wide scientific and industrial attention as interesting classes of compounds because of their outstanding characteristics such as; low toxicity, biodegradability, effectiveness at extreme pH, temperature, alkalinity/acidity and ionic strength, effectiveness at low concentration, excellent emulsification/solubilisation activity, can be synthesised by renewable/waste substrates and have the ability to act as antimicrobial as well as anticancer treating agents. However, due to certain technical and economic factors which one of them includes; the pathogenicity of biosurfactant-producing strains, the industrial scale production and application of biosurfactants is still an unachieved task. This research has been designed to identify new/safe non-pathogenic biosurfactant producing bacteria and to improve their rate of biosurfactant production with the aim of introducing them as biological factories and their biosurfactants can be alternative raw materials in the production of household and health care products. A total of 45 bacterial isolates obtained from two oil-contaminated mangrove soil samples collected from Nigeria, have been screened so far for their ability to produce biosurfactants. Screening for biosurfactant production was done in three selected cultivation media; nutrient broth, mineral salt medium+2% glucose and nutrient broth+3% glycerol and positive results were verified by measuring the reduction in surface tension of cell-free culture supernatant using the Du-Noug ring method. Out of the 45 isolates, 12 have turned out as positive biosurfactant producers. The future plans of the research would include; molecular identification of the positive isolates, improving their rate of biosurfactant production using wastes materials like animal fat and glycerol, characterisation of the biosurfactant produced and to determine their cytotoxicity, antimicrobial, anticancer and emulsification properties. The successful identification of safe biosurfactant- producing microbial strains with the ability to produce sufficient amount of surfactant could create a huge opportunity of replacing the synthetic surfactants with the better biosurfactants in all applicable industrial processes.en
dc.language.isoenen
dc.relation.urlhttp://www.setforbritain.org.uk/index.aspen
dc.relation.urlhttp://www.tees.ac.uk/sections/international/news_story.cfm?story_id=4611&this_issue_title=March%202014&this_issue=250en
dc.titleOptimisation of biosurfactants produced by non-pathogenic bacteria from environmental samplesen
dc.typePresentationen
dc.contributor.departmentTeesside University. Technology Futures Institute.en
dc.identifier.conferenceSET for BRITAIN 2014 poster competition at the House of Commons, organised by Parliamentary and Scientific Committee. London March 17 2014en
or.citation.harvardChibuzo I. Uzoigwe, Pattanathu K. S. M. Rahman, Christopher J. Ennis, and James G. Burgess, (2014). Optimisation of biosurfactants produced by non-pathogenic bacteria from environmental samples. Presented at SET for BRITAIN 2014 poster competition at the House of Commons, London on 17th March 2014 organised by Parliamentary and Scientific Committeeen
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