Changes to soil bacterial profiles as a result of Sus scrofa domesticus decomposition

Hdl Handle:
http://hdl.handle.net/10149/338553
Title:
Changes to soil bacterial profiles as a result of Sus scrofa domesticus decomposition
Authors:
Olakanye, A. O. (Ayodeji); Thompson, T. (Tim); Ralebitso-Senior, T. K. (Theresia Komang)
Affiliation:
Teesside University. Technology Futures Institute.
Citation:
Olakanye, A. O., Thompson, T., Ralebitso-Senior, T. K. (2014) 'Changes to soil bacterial profiles as a result of Sus scrofa domesticus decomposition' Forensic Science International; 245: pp101–106
Publisher:
Elsevier
Journal:
Forensic Science International
Issue Date:
Dec-2014
URI:
http://hdl.handle.net/10149/338553
DOI:
10.1016/j.forsciint.2014.10.002
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S0379073814004083
Abstract:
The importance of cadaver decomposition knowledge for clandestine grave location cannot be over emphasised. Notwithstanding this, only a limited understanding is available on the resulting soil microbial community dynamics. To address this paucity, a pig leg (Sus scrofa domesticus; 5 kg) was buried in freshly weighed (20 kg) sandy loamy soil in a sealed microcosm (40 cm height) in parallel with a soil only control. Both microcosms were perforated nine times at equal distances and maintained outside. Soil samples were collected through these perforations from the top (0–10 cm), middle (10–20 cm) and bottom (20–30 cm) segments every three days for the first two weeks, and then weekly up to 14 weeks. PCR-DGGE gels quantified by 1D Phoretix showed increases in the cumulative soil community richness values of 43, 66 and 106 for the top, middle and bottom segments, respectively, in the presence of Sus scrofa domesticus. Shannon–Wiener’s (H0) and Simpon’s (D) indices confirmed corresponding species diversity increases in the middle (H0 = 1.58–2.33; D = 0.79–0.91) and bottom (H0 = 2.48–3.16; D = 0.85– 0.95) depths between days 10 and 71 compared with the control. In contrast, similar evenness was recorded for all segments in both the Sus scrofa domesticus and control soils.
Type:
Article
Language:
en
Keywords:
Cadaver decomposition; Soil bacterial community; Sus scrofa domesticus; PCR-DGGE
ISSN:
03790738
Rights:
Author can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo [Accessed: 19/01/2015]

Full metadata record

DC FieldValue Language
dc.contributor.authorOlakanye, A. O. (Ayodeji)en
dc.contributor.authorThompson, T. (Tim)en
dc.contributor.authorRalebitso-Senior, T. K. (Theresia Komang)en
dc.date.accessioned2015-01-19T14:22:36Z-
dc.date.available2015-01-19T14:22:36Z-
dc.date.issued2014-12-
dc.identifier.citationChanges to soil bacterial profiles as a result of Sus scrofa domesticus decomposition 2014, 245:101 Forensic Science Internationalen
dc.identifier.issn03790738-
dc.identifier.doi10.1016/j.forsciint.2014.10.002-
dc.identifier.urihttp://hdl.handle.net/10149/338553-
dc.description.abstractThe importance of cadaver decomposition knowledge for clandestine grave location cannot be over emphasised. Notwithstanding this, only a limited understanding is available on the resulting soil microbial community dynamics. To address this paucity, a pig leg (Sus scrofa domesticus; 5 kg) was buried in freshly weighed (20 kg) sandy loamy soil in a sealed microcosm (40 cm height) in parallel with a soil only control. Both microcosms were perforated nine times at equal distances and maintained outside. Soil samples were collected through these perforations from the top (0–10 cm), middle (10–20 cm) and bottom (20–30 cm) segments every three days for the first two weeks, and then weekly up to 14 weeks. PCR-DGGE gels quantified by 1D Phoretix showed increases in the cumulative soil community richness values of 43, 66 and 106 for the top, middle and bottom segments, respectively, in the presence of Sus scrofa domesticus. Shannon–Wiener’s (H0) and Simpon’s (D) indices confirmed corresponding species diversity increases in the middle (H0 = 1.58–2.33; D = 0.79–0.91) and bottom (H0 = 2.48–3.16; D = 0.85– 0.95) depths between days 10 and 71 compared with the control. In contrast, similar evenness was recorded for all segments in both the Sus scrofa domesticus and control soils.en
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0379073814004083en
dc.rightsAuthor can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo [Accessed: 19/01/2015]en
dc.subjectCadaver decompositionen
dc.subjectSoil bacterial communityen
dc.subjectSus scrofa domesticusen
dc.subjectPCR-DGGEen
dc.titleChanges to soil bacterial profiles as a result of Sus scrofa domesticus decompositionen
dc.typeArticleen
dc.contributor.departmentTeesside University. Technology Futures Institute.en
dc.identifier.journalForensic Science Internationalen
or.citation.harvardOlakanye, A. O., Thompson, T., Ralebitso-Senior, T. K. (2014) 'Changes to soil bacterial profiles as a result of Sus scrofa domesticus decomposition' Forensic Science International; 245: pp101–106-
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