Microbioreactor with Photodiode Detection for Monitoring Intracellular GFPUV Expression in E. coli

Hdl Handle:
http://hdl.handle.net/10149/346238
Title:
Microbioreactor with Photodiode Detection for Monitoring Intracellular GFPUV Expression in E. coli
Authors:
Pasirayi, G. (Godfrey); Islam, M. (Meezanul); Scott, S. M. (Simon); O'Hare, W. T. (Liam); Bateson, S. (Simon); Ali, Z. (Zulfiqur)
Affiliation:
Teesside University. Technology Futures Institute.
Citation:
Pasirayi, G., Islam, M., Scott, S. M., O'Hare, W. T., Bateson, S. (2014) 'Microbioreactor with Photodiode Detection for Monitoring Intracellular GFPUV Expression in E. coli' Micro and Nanosystems; 6 (2): 87-94
Publisher:
Bentham Science Publishers
Journal:
Micro and Nanosystems
Issue Date:
2014
URI:
http://hdl.handle.net/10149/346238
DOI:
10.2174/187640290602141127114324#sthash.QCUJLlFh.dpuf
Additional Links:
http://eurekaselect.com/126376
Abstract:
A prototype membrane aerated polydimethylsiloxane (PDMS) microbioreactor integrated with a photodiode detector was developed for monitoring intracellular green fluorescent protein ultraviolet (GFPUV) expression in Escherichia coli. The developed system is compact, simple and inexpensive and ideal for economical on chip detection of fluorophores in aqueous solution and intracellular GFPUV expression. The detection limit for cell free GFPUV was found to be 4.8 x 10-8 M. Intracellular GFPUV expression in E. coli cells was monitored for 12 h and the lowest detectable signal was recorded from a cell concentration of 2.7 x 107cells/mL with signal to noise ratio (SNR) value of 3. The performance of the photodiode detector was benchmarked with a CCD spectrophotometer and results showed favorable comparison. A miniaturized microbioreactor for monitoring intracellular dynamics in real time is demonstrated. - See more at: http://eurekaselect.com/126376#sthash.QCUJLlFh.dpuf
Type:
Article
Language:
en
Keywords:
Fluorescence; intracellular GFPUV; microbioreactor; photodiode
ISSN:
1876-4029
EISSN:
1876-4037
Rights:
Subject to 12 month embargo, author can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo [Accessed: 06/03/2015]

Full metadata record

DC FieldValue Language
dc.contributor.authorPasirayi, G. (Godfrey)en
dc.contributor.authorIslam, M. (Meezanul)en
dc.contributor.authorScott, S. M. (Simon)en
dc.contributor.authorO'Hare, W. T. (Liam)en
dc.contributor.authorBateson, S. (Simon)en
dc.contributor.authorAli, Z. (Zulfiqur)en
dc.date.accessioned2015-03-06T10:46:21Zen
dc.date.available2015-03-06T10:46:21Zen
dc.date.issued2014en
dc.identifier.citationMicro and Nanosystems; 6 (2): 87-94en
dc.identifier.issn1876-4029en
dc.identifier.doi10.2174/187640290602141127114324#sthash.QCUJLlFh.dpufen
dc.identifier.urihttp://hdl.handle.net/10149/346238en
dc.description.abstractA prototype membrane aerated polydimethylsiloxane (PDMS) microbioreactor integrated with a photodiode detector was developed for monitoring intracellular green fluorescent protein ultraviolet (GFPUV) expression in Escherichia coli. The developed system is compact, simple and inexpensive and ideal for economical on chip detection of fluorophores in aqueous solution and intracellular GFPUV expression. The detection limit for cell free GFPUV was found to be 4.8 x 10-8 M. Intracellular GFPUV expression in E. coli cells was monitored for 12 h and the lowest detectable signal was recorded from a cell concentration of 2.7 x 107cells/mL with signal to noise ratio (SNR) value of 3. The performance of the photodiode detector was benchmarked with a CCD spectrophotometer and results showed favorable comparison. A miniaturized microbioreactor for monitoring intracellular dynamics in real time is demonstrated. - See more at: http://eurekaselect.com/126376#sthash.QCUJLlFh.dpufen
dc.language.isoenen
dc.publisherBentham Science Publishersen
dc.relation.urlhttp://eurekaselect.com/126376en
dc.rightsSubject to 12 month embargo, author can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo [Accessed: 06/03/2015]en
dc.subjectFluorescenceen
dc.subjectintracellular GFPUVen
dc.subjectmicrobioreactoren
dc.subjectphotodiodeen
dc.titleMicrobioreactor with Photodiode Detection for Monitoring Intracellular GFPUV Expression in E. colien
dc.typeArticleen
dc.identifier.eissn1876-4037en
dc.contributor.departmentTeesside University. Technology Futures Institute.en
dc.identifier.journalMicro and Nanosystemsen
or.citation.harvardPasirayi, G., Islam, M., Scott, S. M., O'Hare, W. T., Bateson, S. (2014) 'Microbioreactor with Photodiode Detection for Monitoring Intracellular GFPUV Expression in E. coli' Micro and Nanosystems; 6 (2): 87-94en
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