Soil fungal community shift evaluation as a potential cadaver decomposition indicator

Hdl Handle:
http://hdl.handle.net/10149/581790
Title:
Soil fungal community shift evaluation as a potential cadaver decomposition indicator
Authors:
Chimutsa, M. (Monica); Olakanye, A. O. (Ayodeji); Thompson, T. J. U. (Tim); Ralebitso-Senior, T. K. (Theresia Komang)
Affiliation:
Teesside University. Technology Futures Institute.
Citation:
Chimutsa, M., Olakanye, A. O., Thompson, T. J. U., Ralebitso-Senior, T. K. (2015) 'Soil fungal community shift evaluation as a potential cadaver decomposition indicator' Forensic Science International; 257:155
Publisher:
Elsevier
Journal:
Forensic Science International
Issue Date:
Dec-2015 ; 17-Aug-2015
URI:
http://hdl.handle.net/10149/581790
DOI:
10.1016/j.forsciint.2015.08.005
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S0379073815003473
Abstract:
Fungi metabolise organic matter in situ and so alter both the bio-/physico-chemical properties and microbial community structure of the ecosystem. In particular, they are responsible reportedly for specific stages of decomposition. Therefore, this study aimed to extend previous bacteria-based forensic ecogenomics research by investigating soil fungal community and cadaver decomposition interactions in microcosms with garden soil (20 kg, fresh weight) and domestic pig (Sus scrofa domesticus) carcass (5 kg, leg). Soil samples were collected at depths of 0–10 cm, 10–20 cm and 20–30 cm on days 3, 28 and 77 in the absence (control −Pg) and presence (experimental +Pg) of Sus scrofa domesticus and used for total DNA extraction and nested polymerase chain reaction and denaturing gradient gel electrophoresis (PCR–DGGE) profiling of the 18S rRNA gene. The Shannon–Wiener (H′) community diversity indices were 1.25 ± 0.21 and 1.49 ± 0.30 for the control and experimental microcosms, respectively, while comparable Simpson species dominance (S) values were 0.65 ± 0.109 and 0.75 ± 0.015. Generally, and in contrast to parallel studies of the bacterial 16S rRNA and 16S rDNA profiles, statistical analysis (t-test) of the 18S dynamics showed no mathematically significant shifts in fungal community diversity (H′; p = 0.142) and dominance (S; p = 0.392) during carcass decomposition, necessitating further investigations.
Type:
Article
Language:
en
ISSN:
03790738
Rights:
Following 12 month embargo author can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo [Accessed: 05/11/2015]

Full metadata record

DC FieldValue Language
dc.contributor.authorChimutsa, M. (Monica)en
dc.contributor.authorOlakanye, A. O. (Ayodeji)en
dc.contributor.authorThompson, T. J. U. (Tim)en
dc.contributor.authorRalebitso-Senior, T. K. (Theresia Komang)en
dc.date.accessioned2015-11-05T11:46:46Zen
dc.date.available2015-11-05T11:46:46Zen
dc.date.issued2015-12en
dc.date.issued2015-08-17en
dc.identifier.citationForensic Science International; 257:155en
dc.identifier.issn03790738en
dc.identifier.doi10.1016/j.forsciint.2015.08.005en
dc.identifier.urihttp://hdl.handle.net/10149/581790en
dc.description.abstractFungi metabolise organic matter in situ and so alter both the bio-/physico-chemical properties and microbial community structure of the ecosystem. In particular, they are responsible reportedly for specific stages of decomposition. Therefore, this study aimed to extend previous bacteria-based forensic ecogenomics research by investigating soil fungal community and cadaver decomposition interactions in microcosms with garden soil (20 kg, fresh weight) and domestic pig (Sus scrofa domesticus) carcass (5 kg, leg). Soil samples were collected at depths of 0–10 cm, 10–20 cm and 20–30 cm on days 3, 28 and 77 in the absence (control −Pg) and presence (experimental +Pg) of Sus scrofa domesticus and used for total DNA extraction and nested polymerase chain reaction and denaturing gradient gel electrophoresis (PCR–DGGE) profiling of the 18S rRNA gene. The Shannon–Wiener (H′) community diversity indices were 1.25 ± 0.21 and 1.49 ± 0.30 for the control and experimental microcosms, respectively, while comparable Simpson species dominance (S) values were 0.65 ± 0.109 and 0.75 ± 0.015. Generally, and in contrast to parallel studies of the bacterial 16S rRNA and 16S rDNA profiles, statistical analysis (t-test) of the 18S dynamics showed no mathematically significant shifts in fungal community diversity (H′; p = 0.142) and dominance (S; p = 0.392) during carcass decomposition, necessitating further investigations.en
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0379073815003473en
dc.rightsFollowing 12 month embargo author can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo [Accessed: 05/11/2015]en
dc.titleSoil fungal community shift evaluation as a potential cadaver decomposition indicatoren
dc.typeArticleen
dc.contributor.departmentTeesside University. Technology Futures Institute.en
dc.identifier.journalForensic Science Internationalen
or.citation.harvardChimutsa, M., Olakanye, A. O., Thompson, T. J. U., Ralebitso-Senior, T. K. (2015) 'Soil fungal community shift evaluation as a potential cadaver decomposition indicator' Forensic Science International; 257:155en
dc.date.accepted2015-08-08en
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