Impedimetric measurements for monitoring avidin-biotin interaction on self-assembled monolayer

Hdl Handle:
http://hdl.handle.net/10149/93831
Title:
Impedimetric measurements for monitoring avidin-biotin interaction on self-assembled monolayer
Authors:
Pruneanu, S. (Stela); Boughriet, A.; Henderson, A. P. (Andrew); Malins, C.; Ali, Z. (Zulfiqur); Olenic, L. (Liliana)
Affiliation:
Teesside University. School of Science and Technology.
Citation:
Pruneanu, S. et al. (2008) 'Impedimetric measurements for monitoring avidin-biotin interaction on self-assembled monolayer', Particulate Science and Technology, 26 (2), pp.136-144.
Publisher:
Taylor & Francis
Journal:
Particulate Science and Technology
Issue Date:
Mar-2008
URI:
http://hdl.handle.net/10149/93831
DOI:
10.1080/02726350801890870
Abstract:
Avidin-biotin has been controllable immobilized on the surface of gold electrodes using mercaptopropionic acid as self-assembled monolayer. Electrochemical impedance spectroscopy (EIS) was employed to investigate the changes that appear at the electrode surface in the presence of a redox mediator, K3[Fe(CN)6]. An electrical model more complex than that in other studies was used to interpret the EIS measurements (Randles circuit). This model is very useful because it takes into consideration both the transfer of electrons at the electrode/electrolyte interface and the diffusion of redox species through the double layer. The model allowed us to determine some important parameters like solution resistance Rsol, charge-transfer resistance Rct, double-layer capacitance Cdl, Warburg resistance RW, and the diffusion time constant τ. The EIS results proved that immobilization of avidin-biotin increased the charge-transfer resistance Rct, due to the insulating character of these molecules.
Type:
Article
Language:
en
Keywords:
avidin; biotin; impedance spectroscopy; self-assembled monolayer
ISSN:
0272-6351
Rights:
Subject to restrictions, author can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 08/03/2010]
Citation Count:
0 [Web of Science and Scopus, 08/03/2010]

Full metadata record

DC FieldValue Language
dc.contributor.authorPruneanu, S. (Stela)en
dc.contributor.authorBoughriet, A.en
dc.contributor.authorHenderson, A. P. (Andrew)en
dc.contributor.authorMalins, C.en
dc.contributor.authorAli, Z. (Zulfiqur)en
dc.contributor.authorOlenic, L. (Liliana)en
dc.date.accessioned2010-03-08T13:13:41Z-
dc.date.available2010-03-08T13:13:41Z-
dc.date.issued2008-03-
dc.identifier.citationParticulate Science and Technology; 26 (2): 136 - 144en
dc.identifier.issn0272-6351-
dc.identifier.doi10.1080/02726350801890870-
dc.identifier.urihttp://hdl.handle.net/10149/93831-
dc.description.abstractAvidin-biotin has been controllable immobilized on the surface of gold electrodes using mercaptopropionic acid as self-assembled monolayer. Electrochemical impedance spectroscopy (EIS) was employed to investigate the changes that appear at the electrode surface in the presence of a redox mediator, K3[Fe(CN)6]. An electrical model more complex than that in other studies was used to interpret the EIS measurements (Randles circuit). This model is very useful because it takes into consideration both the transfer of electrons at the electrode/electrolyte interface and the diffusion of redox species through the double layer. The model allowed us to determine some important parameters like solution resistance Rsol, charge-transfer resistance Rct, double-layer capacitance Cdl, Warburg resistance RW, and the diffusion time constant τ. The EIS results proved that immobilization of avidin-biotin increased the charge-transfer resistance Rct, due to the insulating character of these molecules.en
dc.language.isoenen
dc.publisherTaylor & Francisen
dc.rightsSubject to restrictions, author can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 08/03/2010]en
dc.subjectavidinen
dc.subjectbiotinen
dc.subjectimpedance spectroscopyen
dc.subjectself-assembled monolayeren
dc.titleImpedimetric measurements for monitoring avidin-biotin interaction on self-assembled monolayeren
dc.typeArticleen
dc.contributor.departmentTeesside University. School of Science and Technology.en
dc.identifier.journalParticulate Science and Technologyen
ref.citationcount0 [Web of Science and Scopus, 08/03/2010]en
or.citation.harvardPruneanu, S. et al. (2008) 'Impedimetric measurements for monitoring avidin-biotin interaction on self-assembled monolayer', Particulate Science and Technology, 26 (2), pp.136-144.-
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