Development of a simple and low cost microbioreactor for high-throughput bioprocessing

Hdl Handle:
http://hdl.handle.net/10149/93875
Title:
Development of a simple and low cost microbioreactor for high-throughput bioprocessing
Authors:
Rahman, P. K. S. M. (Pattanathu); Pasirayi, G. (Godfrey); Auger, V. (Vincent); Ali, Z. (Zulfiqur)
Affiliation:
University of Teesside. Technology Futures Institute
Citation:
Rahman, K. S. M. et al. (2009) 'Development of a simple and low cost microbioreactor for high-throughput bioprocessing', Biotechnology Letters, 31 (2), pp.209-214.
Publisher:
Springer Verlag
Journal:
Biotechnology Letters
Issue Date:
Feb-2009
URI:
http://hdl.handle.net/10149/93875
DOI:
10.1007/s10529-008-9853-8
Abstract:
A simple microbioreactor for high-throughput bioprocessing made from low cost polymer polytetrafluoroethylene (PTFE) tubes with a working volume of 1.5 ml is described. We have developed a microfluidic system that handles a small population of cells of a model microorganism, Pseudomonas aeruginosa DS10-129. Under the conditions of the microbioreactor, the organism produced extracellular secondary metabolites by using nutrient broth modified with glycerol. Pyocyanins were isolated from the fermented medium as a metabolite of interest. Antibiotic properties of pyocyanin were effective against a number of microorganisms such as Staphylococcus aureus, S. epidermis, Bacillus subtilis, Micrococcus luteus and Saccharomyces cerevisiae. Batch fermentation of the model organism in the microbioreactor was compared to shake-flask and conventional bench fermenter methods. Results obtained from the microbioreactor compared favourably with the conventional processes.
Type:
Article
Language:
en
Keywords:
antimicrobial activity; biosurfactant; fermentation; high-throughput; pyocyanin; microbioreactor
ISSN:
0141-5492; 1573-6776
Rights:
Author can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 08/03/2010]

Full metadata record

DC FieldValue Language
dc.contributor.authorRahman, P. K. S. M. (Pattanathu)en
dc.contributor.authorPasirayi, G. (Godfrey)en
dc.contributor.authorAuger, V. (Vincent)en
dc.contributor.authorAli, Z. (Zulfiqur)en
dc.date.accessioned2010-03-08T14:40:15Zen
dc.date.available2010-03-08T14:40:15Zen
dc.date.issued2009-02en
dc.identifier.citationBiotechnology Letters; 31 (2): 209-214en
dc.identifier.issn0141-5492en
dc.identifier.issn1573-6776en
dc.identifier.doi10.1007/s10529-008-9853-8en
dc.identifier.urihttp://hdl.handle.net/10149/93875en
dc.description.abstractA simple microbioreactor for high-throughput bioprocessing made from low cost polymer polytetrafluoroethylene (PTFE) tubes with a working volume of 1.5 ml is described. We have developed a microfluidic system that handles a small population of cells of a model microorganism, Pseudomonas aeruginosa DS10-129. Under the conditions of the microbioreactor, the organism produced extracellular secondary metabolites by using nutrient broth modified with glycerol. Pyocyanins were isolated from the fermented medium as a metabolite of interest. Antibiotic properties of pyocyanin were effective against a number of microorganisms such as Staphylococcus aureus, S. epidermis, Bacillus subtilis, Micrococcus luteus and Saccharomyces cerevisiae. Batch fermentation of the model organism in the microbioreactor was compared to shake-flask and conventional bench fermenter methods. Results obtained from the microbioreactor compared favourably with the conventional processes.en
dc.language.isoenen
dc.publisherSpringer Verlagen
dc.rightsAuthor can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 08/03/2010]en
dc.subjectantimicrobial activityen
dc.subjectbiosurfactanten
dc.subjectfermentationen
dc.subjecthigh-throughputen
dc.subjectpyocyaninen
dc.subjectmicrobioreactoren
dc.titleDevelopment of a simple and low cost microbioreactor for high-throughput bioprocessingen
dc.typeArticleen
dc.contributor.departmentUniversity of Teesside. Technology Futures Instituteen
dc.identifier.journalBiotechnology Lettersen
or.citation.harvardRahman, K. S. M. et al. (2009) 'Development of a simple and low cost microbioreactor for high-throughput bioprocessing', Biotechnology Letters, 31 (2), pp.209-214.en
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