Hairpin-mediated down-regulation of the urea cycle enzyme argininosuccinate lyase in Agaricus bisporus

Hdl Handle:
http://hdl.handle.net/10149/94317
Title:
Hairpin-mediated down-regulation of the urea cycle enzyme argininosuccinate lyase in Agaricus bisporus
Authors:
Eastwood, D. C. (Daniel); Challen, M. P. (Michael); Zhang, C. (Cunjin); Jenkins, H. (Huw); Henderson, J. (Janey); Burton, K. S. (Kerry)
Affiliation:
University of Teesside. School of Science and Technology.
Citation:
Eastwood, D. C. et al. (2008) 'Hairpin-mediated down-regulation of the urea cycle enzyme argininosuccinate lyase in Agaricus bisporus', Mycological Research, 112 (6), pp.708-716.
Publisher:
Elsevier
Journal:
Mycological Research
Issue Date:
Jun-2008
URI:
http://hdl.handle.net/10149/94317
DOI:
10.1016/j.mycres.2008.01.009
Abstract:
A double-stranded (ds) RNA hairpin-mediated down-regulation system was developed for the cultivated mushroom Agaricus bisporus, and the role of the urea cycle enzyme argininosuccinate lyase (asl) in mushroom post-harvest development was investigated. Hairpin expression vectors were constructed to initiate down-regulation of asl and introduced into A. bisporus by Agrobacterium tumefaciens-mediated transformation. Transcripts of asl were significantly reduced (93.1 and 99.9 %) in two transformants and hairpin vector transgene sequences were maintained throughout sporophore development. Single and multiple hairpin integration events were observed in Southern analysis. Transformants with down-regulated asl exhibited reduced yield and cap expansion during post-harvest sporophore development. There were no detectable differences in urea levels between the hairpin-transformed and control strains. This is the first report of reduced gene expression resulting from the introduction of dsRNA hairpins in A. bisporus and the applications of this technology will facilitate functional studies in the mushroom.
Type:
Article
Language:
en
Keywords:
agaricus bisporus; agrobacterium-mediated transformation; argininosuccinate lyase; hairpin; post-harvest; RNA interference; urea cycle
ISSN:
0953-7562; 1469-8102
Rights:
Author can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 16/03/2010]
Citation Count:
6 [Scopus, 16/03/2010]

Full metadata record

DC FieldValue Language
dc.contributor.authorEastwood, D. C. (Daniel)en
dc.contributor.authorChallen, M. P. (Michael)en
dc.contributor.authorZhang, C. (Cunjin)en
dc.contributor.authorJenkins, H. (Huw)en
dc.contributor.authorHenderson, J. (Janey)en
dc.contributor.authorBurton, K. S. (Kerry)en
dc.date.accessioned2010-03-16T12:04:57Z-
dc.date.available2010-03-16T12:04:57Z-
dc.date.issued2008-06-
dc.identifier.citationMycological Research; 112 (6): 708-716en
dc.identifier.issn0953-7562-
dc.identifier.issn1469-8102-
dc.identifier.doi10.1016/j.mycres.2008.01.009-
dc.identifier.urihttp://hdl.handle.net/10149/94317-
dc.description.abstractA double-stranded (ds) RNA hairpin-mediated down-regulation system was developed for the cultivated mushroom Agaricus bisporus, and the role of the urea cycle enzyme argininosuccinate lyase (asl) in mushroom post-harvest development was investigated. Hairpin expression vectors were constructed to initiate down-regulation of asl and introduced into A. bisporus by Agrobacterium tumefaciens-mediated transformation. Transcripts of asl were significantly reduced (93.1 and 99.9 %) in two transformants and hairpin vector transgene sequences were maintained throughout sporophore development. Single and multiple hairpin integration events were observed in Southern analysis. Transformants with down-regulated asl exhibited reduced yield and cap expansion during post-harvest sporophore development. There were no detectable differences in urea levels between the hairpin-transformed and control strains. This is the first report of reduced gene expression resulting from the introduction of dsRNA hairpins in A. bisporus and the applications of this technology will facilitate functional studies in the mushroom.en
dc.language.isoenen
dc.publisherElsevieren
dc.rightsAuthor can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 16/03/2010]en
dc.subjectagaricus bisporusen
dc.subjectagrobacterium-mediated transformationen
dc.subjectargininosuccinate lyaseen
dc.subjecthairpinen
dc.subjectpost-harvesten
dc.subjectRNA interferenceen
dc.subjecturea cycleen
dc.titleHairpin-mediated down-regulation of the urea cycle enzyme argininosuccinate lyase in Agaricus bisporusen
dc.typeArticleen
dc.contributor.departmentUniversity of Teesside. School of Science and Technology.en
dc.identifier.journalMycological Researchen
ref.citationcount6 [Scopus, 16/03/2010]en
or.citation.harvardEastwood, D. C. et al. (2008) 'Hairpin-mediated down-regulation of the urea cycle enzyme argininosuccinate lyase in Agaricus bisporus', Mycological Research, 112 (6), pp.708-716.-
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