Characterization of corrosive bacterial consortia isolated from petroleum-product-transporting pipelines

Hdl Handle:
http://hdl.handle.net/10149/95321
Title:
Characterization of corrosive bacterial consortia isolated from petroleum-product-transporting pipelines
Authors:
Rajasekar, A. (Aruliah); Anandkumar, B. (Balakrishnan); Maruthamuthu, S. (Sundaram); Ting, Y-P. (Yen-Peng); Rahman, P. K. S. M. (Pattanathu)
Affiliation:
University of Teesside. Technology Futures Institute
Citation:
Rajasekar, A. et al. (2010) 'Characterization of corrosive bacterial consortia isolated from petroleum-product-transporting pipelines', Applied Microbiology and Biotechnology, 85 (4), pp.1175-1188.
Publisher:
Springer Verlag
Journal:
Applied Microbiology and Biotechnology
Issue Date:
Jan-2010
URI:
http://hdl.handle.net/10149/95321
DOI:
10.1007/s00253-009-2289-9
Abstract:
Microbiologically influenced corrosion is a problem commonly encountered in facilities in the oil and gas industries. The present study describes bacterial enumeration and identification in diesel and naphtha pipelines located in the northwest and southwest region in India, using traditional cultivation technique and 16S rDNA gene sequencing. Phylogenetic analysis of 16S rRNA sequences of the isolates was carried out, and the samples obtained from the diesel and naphtha-transporting pipelines showed the occurrence of 11 bacterial species namely Serratia marcescens ACE2, Bacillus subtilis AR12, Bacillus cereus ACE4, Pseudomonas aeruginosa AI1, Klebsiella oxytoca ACP, Pseudomonas stutzeri AP2, Bacillus litoralis AN1, Bacillus sp., Bacillus pumilus AR2, Bacillus carboniphilus AR3, and Bacillus megaterium AR4. Sulfate-reducing bacteria were not detected in samples from both pipelines. The dominant bacterial species identified in the petroleum pipeline samples were B. cereus and S. marcescens in the diesel and naphtha pipelines, respectively. Therefore, several types of bacteria may be involved in biocorrosion arising from natural biofilms that develop in industrial facilities. In addition, localized (pitting) corrosion of the pipeline steel in the presence of the consortia was observed by scanning electron microscopy analysis. The potential role of each species in biofilm formation and steel corrosion is discussed.
Type:
Article
Language:
en
Keywords:
16S rDNA analysis; bacterial community; carbon steel API 5 L-X60; microbiologically influenced corrosion; petroleum product pipeline
ISSN:
0175-7598; 1432-0614
Rights:
Author can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 31/03/2010]

Full metadata record

DC FieldValue Language
dc.contributor.authorRajasekar, A. (Aruliah)en
dc.contributor.authorAnandkumar, B. (Balakrishnan)en
dc.contributor.authorMaruthamuthu, S. (Sundaram)en
dc.contributor.authorTing, Y-P. (Yen-Peng)en
dc.contributor.authorRahman, P. K. S. M. (Pattanathu)en
dc.date.accessioned2010-03-31T10:25:19Zen
dc.date.available2010-03-31T10:25:19Zen
dc.date.issued2010-01en
dc.identifier.citationApplied Microbiology and Biotechnology; 85 (4): 1175-1188en
dc.identifier.issn0175-7598en
dc.identifier.issn1432-0614en
dc.identifier.doi10.1007/s00253-009-2289-9en
dc.identifier.urihttp://hdl.handle.net/10149/95321en
dc.description.abstractMicrobiologically influenced corrosion is a problem commonly encountered in facilities in the oil and gas industries. The present study describes bacterial enumeration and identification in diesel and naphtha pipelines located in the northwest and southwest region in India, using traditional cultivation technique and 16S rDNA gene sequencing. Phylogenetic analysis of 16S rRNA sequences of the isolates was carried out, and the samples obtained from the diesel and naphtha-transporting pipelines showed the occurrence of 11 bacterial species namely Serratia marcescens ACE2, Bacillus subtilis AR12, Bacillus cereus ACE4, Pseudomonas aeruginosa AI1, Klebsiella oxytoca ACP, Pseudomonas stutzeri AP2, Bacillus litoralis AN1, Bacillus sp., Bacillus pumilus AR2, Bacillus carboniphilus AR3, and Bacillus megaterium AR4. Sulfate-reducing bacteria were not detected in samples from both pipelines. The dominant bacterial species identified in the petroleum pipeline samples were B. cereus and S. marcescens in the diesel and naphtha pipelines, respectively. Therefore, several types of bacteria may be involved in biocorrosion arising from natural biofilms that develop in industrial facilities. In addition, localized (pitting) corrosion of the pipeline steel in the presence of the consortia was observed by scanning electron microscopy analysis. The potential role of each species in biofilm formation and steel corrosion is discussed.en
dc.language.isoenen
dc.publisherSpringer Verlagen
dc.rightsAuthor can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 31/03/2010]en
dc.subject16S rDNA analysisen
dc.subjectbacterial communityen
dc.subjectcarbon steel API 5 L-X60en
dc.subjectmicrobiologically influenced corrosionen
dc.subjectpetroleum product pipelineen
dc.titleCharacterization of corrosive bacterial consortia isolated from petroleum-product-transporting pipelinesen
dc.typeArticleen
dc.contributor.departmentUniversity of Teesside. Technology Futures Instituteen
dc.identifier.journalApplied Microbiology and Biotechnologyen
or.citation.harvardRajasekar, A. et al. (2010) 'Characterization of corrosive bacterial consortia isolated from petroleum-product-transporting pipelines', Applied Microbiology and Biotechnology, 85 (4), pp.1175-1188.en
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