Production of rhamnolipid biosurfactants by Pseudomonas aeruginosa DS10-129 in a microfluidic bioreactor

Hdl Handle:
http://hdl.handle.net/10149/95334
Title:
Production of rhamnolipid biosurfactants by Pseudomonas aeruginosa DS10-129 in a microfluidic bioreactor
Authors:
Rahman, P. K. S. M. (Pattanathu); Pasirayi, G. (Godfrey); Auger, V. (Vincent); Ali, Z. (Zulfiqur)
Affiliation:
Teesside University. Technology Futures Institute
Citation:
Rahman, P. K. S. M. et al. (2010) 'Production of rhamnolipid biosurfactants by Pseudomonas aeruginosa DS10-129 in a microfluidic bioreactor', Biotechnology and Applied Biochemistry, 55 (1), pp.45-52.
Publisher:
Portland Press
Journal:
Biotechnology and Applied Biochemistry
Issue Date:
2010
URI:
http://hdl.handle.net/10149/95334
DOI:
10.1042/BA20090277
Additional Links:
http://www.babonline.org/bab/055/bab0550045.htm
Abstract:
A low-cost μBR (microbioreactor) made from PTFE [poly(tetrafluoroethylene)] was used to cultivate a model organism, Pseudomonas aeruginosa DS10-129. The progress of bioprocessing was monitored by comparing the growth of the organism in a μBR, a conventional bench scale bioreactor and a shake flask. Under the μBR conditions, the organism produced 23 mg/ml of pyocyanin that had antimicrobial effects against Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas teessidea and Pseudomonas clemancea. Furthermore, it produced a total of 106 μg/ml of effective biosurfactants consisting of dirhamnolipids (RL2) and monorhamnolipids (RL1). The biosurfactants reduced the surface tension of distilled water from 72 to 27.9 mN/m and emulsified kerosene by 71.30%. The pyocyanin and rhamnolipids were produced during the exponential and stationary phases of growth respectively. The results of the μBR were comparable to those obtained using conventional scale methods.
Type:
Article
Language:
en
Keywords:
antimicrobial activity; biosurfactant; high-throughput bioprocessing; microbioreactor; microfluidic bioreactor; rhamnolipid
ISSN:
0885-4513; 1470-8744
Rights:
Subject to restrictions, author can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 31/03/2010]

Full metadata record

DC FieldValue Language
dc.contributor.authorRahman, P. K. S. M. (Pattanathu)en
dc.contributor.authorPasirayi, G. (Godfrey)en
dc.contributor.authorAuger, V. (Vincent)en
dc.contributor.authorAli, Z. (Zulfiqur)en
dc.date.accessioned2010-03-31T10:07:54Zen
dc.date.available2010-03-31T10:07:54Zen
dc.date.issued2010en
dc.identifier.citationBiotechnology and Applied Biochemistry; 55 (1): 45-52en
dc.identifier.issn0885-4513en
dc.identifier.issn1470-8744en
dc.identifier.doi10.1042/BA20090277en
dc.identifier.urihttp://hdl.handle.net/10149/95334en
dc.description.abstractA low-cost μBR (microbioreactor) made from PTFE [poly(tetrafluoroethylene)] was used to cultivate a model organism, Pseudomonas aeruginosa DS10-129. The progress of bioprocessing was monitored by comparing the growth of the organism in a μBR, a conventional bench scale bioreactor and a shake flask. Under the μBR conditions, the organism produced 23 mg/ml of pyocyanin that had antimicrobial effects against Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas teessidea and Pseudomonas clemancea. Furthermore, it produced a total of 106 μg/ml of effective biosurfactants consisting of dirhamnolipids (RL2) and monorhamnolipids (RL1). The biosurfactants reduced the surface tension of distilled water from 72 to 27.9 mN/m and emulsified kerosene by 71.30%. The pyocyanin and rhamnolipids were produced during the exponential and stationary phases of growth respectively. The results of the μBR were comparable to those obtained using conventional scale methods.en
dc.language.isoenen
dc.publisherPortland Pressen
dc.relation.urlhttp://www.babonline.org/bab/055/bab0550045.htmen
dc.rightsSubject to restrictions, author can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 31/03/2010]en
dc.subjectantimicrobial activityen
dc.subjectbiosurfactanten
dc.subjecthigh-throughput bioprocessingen
dc.subjectmicrobioreactoren
dc.subjectmicrofluidic bioreactoren
dc.subjectrhamnolipiden
dc.titleProduction of rhamnolipid biosurfactants by Pseudomonas aeruginosa DS10-129 in a microfluidic bioreactoren
dc.typeArticleen
dc.contributor.departmentTeesside University. Technology Futures Instituteen
dc.identifier.journalBiotechnology and Applied Biochemistryen
or.citation.harvardRahman, P. K. S. M. et al. (2010) 'Production of rhamnolipid biosurfactants by Pseudomonas aeruginosa DS10-129 in a microfluidic bioreactor', Biotechnology and Applied Biochemistry, 55 (1), pp.45-52.en
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