Effect of electrical conditions on an impedimetric immunosensor based on a modified conducting polypyrrole

Hdl Handle:
http://hdl.handle.net/10149/97773
Title:
Effect of electrical conditions on an impedimetric immunosensor based on a modified conducting polypyrrole
Authors:
Hafaid, I. (Imen); Chebil, S. (Syrine); Korri-Youssoufi, H. (Hafsa); Bessueille, F. (François); Errachid, A. (Abdelhamid); Sassi, Z. (Zina); Ali, Z. (Zulfiqur); Abdelghani, A. (Adnane); Jaffrezic-Renault, N. (Nicole)
Affiliation:
Teesside University
Citation:
Hafaid, I. et. al. (2010) 'Effect of electrical conditions on an impedimetric immunosensor based on a modified conducting polypyrrole', Sensors and Actuators B Chemical, 144 (1), pp.323-331.
Publisher:
Elsevier
Journal:
Sensors and Actuators B Chemical
Issue Date:
Jan-2010
URI:
http://hdl.handle.net/10149/97773
DOI:
10.1016/j.snb.2009.08.058
Abstract:
This work has explored the development of impedimetric immunosensors based on a conducting co-polypyrrole film to which a histidine tag antibody has been bound using a complex NTA chelator coordinated with copper as metal. The electrical properties of the copolymer film have been characterized by electrochemical impedance spectroscopy (EIS). The experimental study of EIS was performed over a range of dc voltages from 0 V to -1.4 V (vs. saturated calomel electrode SCE) where the polypyrrole layers transit from their conducting to insulating state and to demonstrate the contribution of the copper complex as redox probe. The electrical behavior of the biomaterial at these various potentials was studied according to an equivalent circuit fitting the experimental parameters. The results indicated that better immunosensor characteristics were obtained at an applied potential of -1400 mV where the reduction of copper occurs. The impedance obtained at this potential shows a linear variation of charge transfer resistance (Rct) with the specific antigen concentration in the range of 0.1-10 ng mL-1 with a detection limit of 0.1 ng mL-1. Moreover, this study demonstrates that these immunosensors exhibit high sensitivity, stability and reproducibility.
Type:
Article
Language:
en
Keywords:
conducting polymer; his-tag antibody; immunosensors; impendance spectroscopy
ISSN:
0925-4005
Rights:
Author can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 30/04/2010]
Citation Count:
0 [Scopus, 30/04/2010]

Full metadata record

DC FieldValue Language
dc.contributor.authorHafaid, I. (Imen)en
dc.contributor.authorChebil, S. (Syrine)en
dc.contributor.authorKorri-Youssoufi, H. (Hafsa)en
dc.contributor.authorBessueille, F. (François)en
dc.contributor.authorErrachid, A. (Abdelhamid)en
dc.contributor.authorSassi, Z. (Zina)en
dc.contributor.authorAli, Z. (Zulfiqur)en
dc.contributor.authorAbdelghani, A. (Adnane)en
dc.contributor.authorJaffrezic-Renault, N. (Nicole)en
dc.date.accessioned2010-04-30T15:39:00Z-
dc.date.available2010-04-30T15:39:00Z-
dc.date.issued2010-01-
dc.identifier.citationSensors and Actuators B Chemical; 144(1):323-331en
dc.identifier.issn0925-4005-
dc.identifier.doi10.1016/j.snb.2009.08.058-
dc.identifier.urihttp://hdl.handle.net/10149/97773-
dc.description.abstractThis work has explored the development of impedimetric immunosensors based on a conducting co-polypyrrole film to which a histidine tag antibody has been bound using a complex NTA chelator coordinated with copper as metal. The electrical properties of the copolymer film have been characterized by electrochemical impedance spectroscopy (EIS). The experimental study of EIS was performed over a range of dc voltages from 0 V to -1.4 V (vs. saturated calomel electrode SCE) where the polypyrrole layers transit from their conducting to insulating state and to demonstrate the contribution of the copper complex as redox probe. The electrical behavior of the biomaterial at these various potentials was studied according to an equivalent circuit fitting the experimental parameters. The results indicated that better immunosensor characteristics were obtained at an applied potential of -1400 mV where the reduction of copper occurs. The impedance obtained at this potential shows a linear variation of charge transfer resistance (Rct) with the specific antigen concentration in the range of 0.1-10 ng mL-1 with a detection limit of 0.1 ng mL-1. Moreover, this study demonstrates that these immunosensors exhibit high sensitivity, stability and reproducibility.en
dc.language.isoenen
dc.publisherElsevieren
dc.rightsAuthor can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 30/04/2010]en
dc.subjectconducting polymeren
dc.subjecthis-tag antibodyen
dc.subjectimmunosensorsen
dc.subjectimpendance spectroscopyen
dc.titleEffect of electrical conditions on an impedimetric immunosensor based on a modified conducting polypyrroleen
dc.typeArticleen
dc.contributor.departmentTeesside Universityen
dc.identifier.journalSensors and Actuators B Chemicalen
ref.citationcount0 [Scopus, 30/04/2010]en
or.citation.harvardHafaid, I. et. al. (2010) 'Effect of electrical conditions on an impedimetric immunosensor based on a modified conducting polypyrrole', Sensors and Actuators B Chemical, 144 (1), pp.323-331.-
All Items in TeesRep are protected by copyright, with all rights reserved, unless otherwise indicated.