Application of the quartz crystal microbalance to the monitoring of Staphylococcus epidermidis antigen-antibody agglutination

Hdl Handle:
http://hdl.handle.net/10149/98013
Title:
Application of the quartz crystal microbalance to the monitoring of Staphylococcus epidermidis antigen-antibody agglutination
Authors:
Pavey, K. D. (Karl); Ali, Z. (Zulfiqur); Olliff, C. J. (Cedric); Paul, F.
Affiliation:
University of Teesside. Division of Biotechnology and Chemical Sciences.
Citation:
Pavey, K. D. et. al. (1999) 'Application of the quartz crystal microbalance to the monitoring of Staphylococcus epidermidis antigen-antibody agglutination', Journal of Pharmaceutical and Biomedical Analysis, 20 (1-2), pp.241-245.
Publisher:
Elsevier
Journal:
Journal of Pharmaceutical and Biomedical Analysis
Issue Date:
Jun-1999
URI:
http://hdl.handle.net/10149/98013
DOI:
10.1016/S0731-7085(99)00026-6
Abstract:
The change in solution properties due to the agglutination of an antigen with its specific antibody has previously been used as a marker of infection. This method has been modified to allow the binding activity between species to be followed using the frequency response of a quartz crystal microbalance (QCM). The Bayston agglutination plate assay for Staphylococcus epidermidis has been modified to allow the electrode of a QCM to act as a direct sensor for the change in solution properties as agglutination occurs. Antibody and antigen were introduced to the crystal surface and the agglutination process was followed as a change in crystal resonant frequency. Serum, known to be infected with the organism, gave a titre of 3.9x10-2% v/v (-118 Hz, ±12 SD, N=9) matching that given by triplicate plate assay. Uninfected serum gave no frequency changes at this concentration, yielding a titre of 2.5x10-2% v/v again matching the plate titre (N=3). Infected serum gave responses 40 times faster then those of the uninfected serum. The piezoelectric quartz crystal method gave a positive or negative diagnosis in <15 min compared with the 24 h required for the plate assay.
Type:
Article
Language:
en
Keywords:
agglutination; quartz crystal microbalance; QCM; rapid diagnosis; staphylococcus epidermidis
ISSN:
0731-7085
Rights:
Author can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 06/05/2010]
Citation Count:
21 [Scopus, 06/05/2010]

Full metadata record

DC FieldValue Language
dc.contributor.authorPavey, K. D. (Karl)en
dc.contributor.authorAli, Z. (Zulfiqur)en
dc.contributor.authorOlliff, C. J. (Cedric)en
dc.contributor.authorPaul, F.en
dc.date.accessioned2010-05-06T07:50:00Z-
dc.date.available2010-05-06T07:50:00Z-
dc.date.issued1999-06-
dc.identifier.citationJournal of Pharmaceutical and Biomedical Analysis; 20(1-2):241-245en
dc.identifier.issn0731-7085-
dc.identifier.doi10.1016/S0731-7085(99)00026-6-
dc.identifier.urihttp://hdl.handle.net/10149/98013-
dc.description.abstractThe change in solution properties due to the agglutination of an antigen with its specific antibody has previously been used as a marker of infection. This method has been modified to allow the binding activity between species to be followed using the frequency response of a quartz crystal microbalance (QCM). The Bayston agglutination plate assay for Staphylococcus epidermidis has been modified to allow the electrode of a QCM to act as a direct sensor for the change in solution properties as agglutination occurs. Antibody and antigen were introduced to the crystal surface and the agglutination process was followed as a change in crystal resonant frequency. Serum, known to be infected with the organism, gave a titre of 3.9x10-2% v/v (-118 Hz, ±12 SD, N=9) matching that given by triplicate plate assay. Uninfected serum gave no frequency changes at this concentration, yielding a titre of 2.5x10-2% v/v again matching the plate titre (N=3). Infected serum gave responses 40 times faster then those of the uninfected serum. The piezoelectric quartz crystal method gave a positive or negative diagnosis in <15 min compared with the 24 h required for the plate assay.en
dc.language.isoenen
dc.publisherElsevieren
dc.rightsAuthor can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 06/05/2010]en
dc.subjectagglutinationen
dc.subjectquartz crystal microbalanceen
dc.subjectQCMen
dc.subjectrapid diagnosisen
dc.subjectstaphylococcus epidermidisen
dc.titleApplication of the quartz crystal microbalance to the monitoring of Staphylococcus epidermidis antigen-antibody agglutinationen
dc.typeArticleen
dc.contributor.departmentUniversity of Teesside. Division of Biotechnology and Chemical Sciences.en
dc.identifier.journalJournal of Pharmaceutical and Biomedical Analysisen
ref.citationcount21 [Scopus, 06/05/2010]en
or.citation.harvardPavey, K. D. et. al. (1999) 'Application of the quartz crystal microbalance to the monitoring of Staphylococcus epidermidis antigen-antibody agglutination', Journal of Pharmaceutical and Biomedical Analysis, 20 (1-2), pp.241-245.-
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