Hdl Handle:
http://hdl.handle.net/10149/98022
Title:
Microbial analysis using Sharma's reaction
Book Title:
Environmental monitoring and remediation technologies
Authors:
Ali, Z. (Zulfiqur); Hodson, B. (Bruce); Timmins, J. (James); Keith, S. (Stephen)
Editors:
Vo-Dinh, T. (Tuan); Spellicy, R. L. (Robert)
Affiliation:
University of Teesside
Citation:
Ali, Z. et. al. (1999) 'Microbial analysis using Sharma's reaction', 1998 environmental monitoring and remediation technologies, Boston, USA, November 2 - 5, in Vo-Dinh, T. and Spellicy, R. L. (eds) Environmental monitoring and remediation technologies. SPIE Society of Photo-Optical Instrumentation Engineers, pp.641-646.
Publisher:
SPIE Society of Photo-Optical Instrumentation Engineers
Conference:
1998 environmental monitoring and remediation technologies, Boston, USA, November 2 - 5, 1998
Issue Date:
Feb-1999
URI:
http://hdl.handle.net/10149/98022
DOI:
10.1117/12.339046
Abstract:
The photoreaction of thionine with NAD(P)H leads to the formation of non-fluorescent semi and lecuothionine. This has been previously used for the development of enzymatic assays. We show that the fluorescence of thionine is quenched in the presence of Nocardia corallina. We ascribe this decrease in fluorescence to the presence of NAD(P)H present on the walls and in the body of the cells as described by Sharma. We therefore demonstrate that it is possible to measure, in situ, NAD(P)H in Nocardia corallina in a simple and inexpensive manner and that this finding extends the analytical application of Sharma's reaction to other organisms.
Type:
Meetings and Proceedings; Book Chapter
Language:
en
Keywords:
microbial analysis; Sharma's reaction; nocardia corallina
Series/Report no.:
3534
ISSN:
0277-786X
Rights:
Author can archive publisher's version/PDF. For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 24/06/2010]
Citation Count:
0 [Scopus, 06/05/2010]

Full metadata record

DC FieldValue Language
dc.contributor.authorAli, Z. (Zulfiqur)en
dc.contributor.authorHodson, B. (Bruce)en
dc.contributor.authorTimmins, J. (James)en
dc.contributor.authorKeith, S. (Stephen)en
dc.contributor.editorVo-Dinh, T. (Tuan)en
dc.contributor.editorSpellicy, R. L. (Robert)en
dc.date.accessioned2010-05-06T09:58:43Z-
dc.date.available2010-05-06T09:58:43Z-
dc.date.issued1999-02-
dc.identifier.issn0277-786X-
dc.identifier.doi10.1117/12.339046-
dc.identifier.urihttp://hdl.handle.net/10149/98022-
dc.description.abstractThe photoreaction of thionine with NAD(P)H leads to the formation of non-fluorescent semi and lecuothionine. This has been previously used for the development of enzymatic assays. We show that the fluorescence of thionine is quenched in the presence of Nocardia corallina. We ascribe this decrease in fluorescence to the presence of NAD(P)H present on the walls and in the body of the cells as described by Sharma. We therefore demonstrate that it is possible to measure, in situ, NAD(P)H in Nocardia corallina in a simple and inexpensive manner and that this finding extends the analytical application of Sharma's reaction to other organisms.en
dc.language.isoenen
dc.publisherSPIE Society of Photo-Optical Instrumentation Engineersen
dc.relation.ispartofseries3534en
dc.rightsAuthor can archive publisher's version/PDF. For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 24/06/2010]en
dc.subjectmicrobial analysisen
dc.subjectSharma's reactionen
dc.subjectnocardia corallinaen
dc.titleMicrobial analysis using Sharma's reactionen
dc.typeMeetings and Proceedingsen
dc.typeBook Chapteren
dc.contributor.departmentUniversity of Teessideen
dc.title.bookEnvironmental monitoring and remediation technologiesen
dc.identifier.conference1998 environmental monitoring and remediation technologies, Boston, USA, November 2 - 5, 1998en
ref.citationcount0 [Scopus, 06/05/2010]en
or.citation.harvardAli, Z. et. al. (1999) 'Microbial analysis using Sharma's reaction', 1998 environmental monitoring and remediation technologies, Boston, USA, November 2 - 5, in Vo-Dinh, T. and Spellicy, R. L. (eds) Environmental monitoring and remediation technologies. SPIE Society of Photo-Optical Instrumentation Engineers, pp.641-646.-
prism.startingPage641-
prism.endingPage646-
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