Electrochemical immobilisation of enzymes. Part 4. - Co-immobilisation of glucose oxidase and ferro/ferricyanide in poly(N-methylpyrrole) films

Hdl Handle:
http://hdl.handle.net/10149/98042
Title:
Electrochemical immobilisation of enzymes. Part 4. - Co-immobilisation of glucose oxidase and ferro/ferricyanide in poly(N-methylpyrrole) films
Authors:
Bartlett, P. N. (Philip); Ali, Z. (Zulfiqur); Eastwick-Field, V. M. (Vanessa)
Affiliation:
University of Bath. School of Chemistry; Brighton Polytechnic. Department of Pharmacy.
Citation:
Bartlett, P. N., Ali, Z. and Eastwick-Field, V. (1992) 'Electrochemical immobilisation of enzymes. Part 4. - Co-immobilisation of glucose oxidase and ferro/ferricyanide in poly(N-methylpyrrole) films', Journal of the Chemical Society Faraday Transactions, 88 (18), pp.2677-2683.
Publisher:
Royal Society of Chemistry
Journal:
Journal of the Chemical Society Faraday Transactions
Issue Date:
1992
URI:
http://hdl.handle.net/10149/98042
DOI:
10.1039/ft9928802677
Abstract:
Glucose oxidase and ferro/ferricyanide can be co-immobilised in poly(N-methylpyrrole) films. The two species compete for incorporation into the films so that on increasing the concentration of enzyme in the growth solution less ferro/ferricyanide is incorporated into the film, and vice versa. The ferro/ferricyanide entrapped within the film acts as a redox mediator for the oxidation of the enzyme at 0.45 V vs. SCE. Studies of the effect of variation of film thickness, glucose concentration, enzyme loading and ferro/ferricyanide loading are consistent with a model in which diffusion and enzyme-catalysed reaction within the film are coupled. Analysis of data from these experiments allows the rate parameters for these processes to be determined. These data are compared with previous results for oxygen mediation in poly(N-methylpyrrole) films.
Type:
Article
Language:
en
Keywords:
electrochemical immobilisation; enzymes; glucose oxidase; ferro/ferricyanide
ISSN:
0956-5000; 1364-5455
Rights:
Author can archive publisher's version/PDF. For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 06/05/2010]
Citation Count:
47 [Scopus, 06/05/2010]

Full metadata record

DC FieldValue Language
dc.contributor.authorBartlett, P. N. (Philip)en
dc.contributor.authorAli, Z. (Zulfiqur)en
dc.contributor.authorEastwick-Field, V. M. (Vanessa)en
dc.date.accessioned2010-05-06T10:36:04Z-
dc.date.available2010-05-06T10:36:04Z-
dc.date.issued1992-
dc.identifier.citationJournal of the Chemical Society Faraday Transactions; 88(18):2677-2683en
dc.identifier.issn0956-5000-
dc.identifier.issn1364-5455-
dc.identifier.doi10.1039/ft9928802677-
dc.identifier.urihttp://hdl.handle.net/10149/98042-
dc.description.abstractGlucose oxidase and ferro/ferricyanide can be co-immobilised in poly(N-methylpyrrole) films. The two species compete for incorporation into the films so that on increasing the concentration of enzyme in the growth solution less ferro/ferricyanide is incorporated into the film, and vice versa. The ferro/ferricyanide entrapped within the film acts as a redox mediator for the oxidation of the enzyme at 0.45 V vs. SCE. Studies of the effect of variation of film thickness, glucose concentration, enzyme loading and ferro/ferricyanide loading are consistent with a model in which diffusion and enzyme-catalysed reaction within the film are coupled. Analysis of data from these experiments allows the rate parameters for these processes to be determined. These data are compared with previous results for oxygen mediation in poly(N-methylpyrrole) films.en
dc.language.isoenen
dc.publisherRoyal Society of Chemistry-
dc.rightsAuthor can archive publisher's version/PDF. For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 06/05/2010]en
dc.subjectelectrochemical immobilisationen
dc.subjectenzymesen
dc.subjectglucose oxidaseen
dc.subjectferro/ferricyanideen
dc.titleElectrochemical immobilisation of enzymes. Part 4. - Co-immobilisation of glucose oxidase and ferro/ferricyanide in poly(N-methylpyrrole) filmsen
dc.typeArticleen
dc.contributor.departmentUniversity of Bath. School of Chemistry; Brighton Polytechnic. Department of Pharmacy.en
dc.identifier.journalJournal of the Chemical Society Faraday Transactionsen
ref.citationcount47 [Scopus, 06/05/2010]en
or.citation.harvardBartlett, P. N., Ali, Z. and Eastwick-Field, V. (1992) 'Electrochemical immobilisation of enzymes. Part 4. - Co-immobilisation of glucose oxidase and ferro/ferricyanide in poly(N-methylpyrrole) films', Journal of the Chemical Society Faraday Transactions, 88 (18), pp.2677-2683.-
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