Effects of rhamnolipids from pseudomonas aeruginosa DS10-129 on luminescent bacteria: Toxicity and modulation of cadmium bioavailability

Hdl Handle:
http://hdl.handle.net/10149/98674
Title:
Effects of rhamnolipids from pseudomonas aeruginosa DS10-129 on luminescent bacteria: Toxicity and modulation of cadmium bioavailability
Authors:
Bondarenko, O. (Olesja); Rahman, P. K. S. M. (Pattanathu); Rahman, T. J. (Thahira); Kahru, A. (Anne); Ivask, A. (Angela)
Affiliation:
Teesside University. Technology Futures Institute
Citation:
Bondarenko, O. et. al. (2010) 'Effects of rhamnolipids from pseudomonas aeruginosa DS10-129 on luminescent bacteria: Toxicity and modulation of cadmium bioavailability', Microbial Ecology, 59(3), pp.588-600.
Publisher:
Springer New York
Journal:
Microbial Ecology
Issue Date:
Apr-2010
URI:
http://hdl.handle.net/10149/98674
DOI:
10.1007/s00248-009-9626-5
Abstract:
In this study, the mixture of mono- and di-rhamnolipids produced by Pseudomonas aeruginosa DS10-129 was characterized for its toxicity and modulatory effects on Cd availability to different bacteria. Gram-negative naturally bioluminescent Vibrio fischeri and recombinant bioluminescent Pseudomonas fluorescens, P. aeruginosa, Escherichia coli, and Gram-positive Bacillus subtilis were used as model organisms. Rhamnolipids reduced the bioluminescence of these bacteria in less than a second of exposure even in relatively low concentrations (30-min EC50 45-167 mg l-1). Toxicity of Cd to Gram-negative bacteria (30-min EC50 values 0.16 mg l-1 for E. coli, 0.96 mg l-1 for P. fluorescens, and 4.4 mg l-1 for V. fischeri) was remarkably (up to 10-fold) reduced in the presence of 50 mg l-1 rhamnolipids. Interestingly, the toxicity of Cd to Gram-positive B. subtilis (30-min EC50 value 0.49 mg l-1) was not affected by rhamnolipids. Rhamnolipids had an effect on desorption of Cd from soil: 40 mg l-1 rhamnolipids increased the water-extracted fraction of Cd twice compared with untreated control. However, this additionally desorbed fraction of Cd remained bound with rhamnolipids and was not available to bacteria. Hence, in carefully chosen concentrations (still effectively complexing heavy metals but not yet toxic to soil bacteria), rhamnolipids could be applied in remediation of polluted areas.
Type:
Article
Language:
en
Keywords:
rhamnolipids; pseudomonas aeruginosa DS10-129; luminescent bacteria; toxicity; modulation; cadmium bioavailability
ISSN:
0095-3628; 1432-184X
Rights:
Author can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 13/05/2010]

Full metadata record

DC FieldValue Language
dc.contributor.authorBondarenko, O. (Olesja)en
dc.contributor.authorRahman, P. K. S. M. (Pattanathu)en
dc.contributor.authorRahman, T. J. (Thahira)en
dc.contributor.authorKahru, A. (Anne)en
dc.contributor.authorIvask, A. (Angela)en
dc.date.accessioned2010-05-13T08:42:05Zen
dc.date.available2010-05-13T08:42:05Zen
dc.date.issued2010-04en
dc.identifier.citationMicrobial Ecology; 59(3):588-600en
dc.identifier.issn0095-3628en
dc.identifier.issn1432-184Xen
dc.identifier.doi10.1007/s00248-009-9626-5en
dc.identifier.urihttp://hdl.handle.net/10149/98674en
dc.description.abstractIn this study, the mixture of mono- and di-rhamnolipids produced by Pseudomonas aeruginosa DS10-129 was characterized for its toxicity and modulatory effects on Cd availability to different bacteria. Gram-negative naturally bioluminescent Vibrio fischeri and recombinant bioluminescent Pseudomonas fluorescens, P. aeruginosa, Escherichia coli, and Gram-positive Bacillus subtilis were used as model organisms. Rhamnolipids reduced the bioluminescence of these bacteria in less than a second of exposure even in relatively low concentrations (30-min EC50 45-167 mg l-1). Toxicity of Cd to Gram-negative bacteria (30-min EC50 values 0.16 mg l-1 for E. coli, 0.96 mg l-1 for P. fluorescens, and 4.4 mg l-1 for V. fischeri) was remarkably (up to 10-fold) reduced in the presence of 50 mg l-1 rhamnolipids. Interestingly, the toxicity of Cd to Gram-positive B. subtilis (30-min EC50 value 0.49 mg l-1) was not affected by rhamnolipids. Rhamnolipids had an effect on desorption of Cd from soil: 40 mg l-1 rhamnolipids increased the water-extracted fraction of Cd twice compared with untreated control. However, this additionally desorbed fraction of Cd remained bound with rhamnolipids and was not available to bacteria. Hence, in carefully chosen concentrations (still effectively complexing heavy metals but not yet toxic to soil bacteria), rhamnolipids could be applied in remediation of polluted areas.en
dc.language.isoenen
dc.publisherSpringer New Yorken
dc.rightsAuthor can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 13/05/2010]en
dc.subjectrhamnolipidsen
dc.subjectpseudomonas aeruginosa DS10-129en
dc.subjectluminescent bacteriaen
dc.subjecttoxicityen
dc.subjectmodulationen
dc.subjectcadmium bioavailabilityen
dc.titleEffects of rhamnolipids from pseudomonas aeruginosa DS10-129 on luminescent bacteria: Toxicity and modulation of cadmium bioavailabilityen
dc.typeArticleen
dc.contributor.departmentTeesside University. Technology Futures Instituteen
dc.identifier.journalMicrobial Ecologyen
or.citation.harvardBondarenko, O. et. al. (2010) 'Effects of rhamnolipids from pseudomonas aeruginosa DS10-129 on luminescent bacteria: Toxicity and modulation of cadmium bioavailability', Microbial Ecology, 59(3), pp.588-600.en
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