The potential of bacterial isolates for emulsification with a range of hydrocarbons

Hdl Handle:
http://hdl.handle.net/10149/98675
Title:
The potential of bacterial isolates for emulsification with a range of hydrocarbons
Authors:
Rahman, P. K. S. M. (Pattanathu); Rahman, T. J. (Thahira); Lakshmanaperumalsamy, P. (Perumalsamy); Marchant, R. (Roger); Banat, I. M. (Ibrahim)
Affiliation:
University of Ulster. School of Biological/Environmental Sciences. Biotechnology Research Group.
Citation:
Rahman, P. K. S. M. et. al. (2003) 'The potential of bacterial isolates for emulsification with a range of hydrocarbons', Acta Biotechnologica, 23 (4), pp.335-345.
Publisher:
Wiley - VCH Verlag
Journal:
Acta Biotechnologica
Issue Date:
2003
URI:
http://hdl.handle.net/10149/98675
DOI:
10.1002/abio.200390043
Abstract:
A study was undertaken to investigate the distribution of biosurfactant producing and crude oil degrading bacteria in the oil contaminated environment. This research revealed that hydrocarbon contaminated sites are the potent sources for oil degraders. Among 32 oil degrading bacteria isolated from ten different oil contaminated sites of gasoline and diesel fuel stations, 80% exhibited biosurfactant production. The quantity and emulsification activity of the biosurfactants varied. Pseudomonas sp. DS10-129 produced a maximum of 7.5 ± 0.4 g/l of biosurfactant with a corresponding reduction in surface tension from 68 mN/m to 29.4 ± 0.7 mN/m at 84 h incubation. The isolates Micrococcus sp. GS2-22, Bacillus sp. DS6-86, Corynebacterium sp. GS5-66, Flavobacterium sp. DS5-73, Pseudomonas sp. DS10-129, Pseudomonas sp. DS9-119 and Acinetobacter sp. DS5-74 emulsified xylene, benzene, n-hexane, Bombay High crude oil, kerosene, gasoline, diesel fuel and olive oil. The first five of the above isolates had the highest emulsification activity and crude oil degradation ability and were selected for the preparation of a mixed bacterial consortium, which was also an efficient biosurfactant producing oil emulsifying and degrading culture. During this study, biosurfactant production and emulsification activity were detected in Moraxella sp., Flavobacterium sp. and in a mixed bacterial consortium, which have not been reported before.
Type:
Article
Language:
en
Keywords:
bacterial isolates; emulsification; hydrocarbons; contamination; oil degraders
ISSN:
0138-4988; 1521-3846
Rights:
Author can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 13/05/2010]
Citation Count:
7 [Scopus, 13/05/2010]

Full metadata record

DC FieldValue Language
dc.contributor.authorRahman, P. K. S. M. (Pattanathu)en
dc.contributor.authorRahman, T. J. (Thahira)en
dc.contributor.authorLakshmanaperumalsamy, P. (Perumalsamy)en
dc.contributor.authorMarchant, R. (Roger)en
dc.contributor.authorBanat, I. M. (Ibrahim)en
dc.date.accessioned2010-05-13T08:58:29Z-
dc.date.available2010-05-13T08:58:29Z-
dc.date.issued2003-
dc.identifier.citationActa Biotechnologica; 23(4):335-345en
dc.identifier.issn0138-4988-
dc.identifier.issn1521-3846-
dc.identifier.doi10.1002/abio.200390043-
dc.identifier.urihttp://hdl.handle.net/10149/98675-
dc.description.abstractA study was undertaken to investigate the distribution of biosurfactant producing and crude oil degrading bacteria in the oil contaminated environment. This research revealed that hydrocarbon contaminated sites are the potent sources for oil degraders. Among 32 oil degrading bacteria isolated from ten different oil contaminated sites of gasoline and diesel fuel stations, 80% exhibited biosurfactant production. The quantity and emulsification activity of the biosurfactants varied. Pseudomonas sp. DS10-129 produced a maximum of 7.5 ± 0.4 g/l of biosurfactant with a corresponding reduction in surface tension from 68 mN/m to 29.4 ± 0.7 mN/m at 84 h incubation. The isolates Micrococcus sp. GS2-22, Bacillus sp. DS6-86, Corynebacterium sp. GS5-66, Flavobacterium sp. DS5-73, Pseudomonas sp. DS10-129, Pseudomonas sp. DS9-119 and Acinetobacter sp. DS5-74 emulsified xylene, benzene, n-hexane, Bombay High crude oil, kerosene, gasoline, diesel fuel and olive oil. The first five of the above isolates had the highest emulsification activity and crude oil degradation ability and were selected for the preparation of a mixed bacterial consortium, which was also an efficient biosurfactant producing oil emulsifying and degrading culture. During this study, biosurfactant production and emulsification activity were detected in Moraxella sp., Flavobacterium sp. and in a mixed bacterial consortium, which have not been reported before.en
dc.language.isoenen
dc.publisherWiley - VCH Verlagen
dc.rightsAuthor can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 13/05/2010]en
dc.subjectbacterial isolatesen
dc.subjectemulsificationen
dc.subjecthydrocarbonsen
dc.subjectcontaminationen
dc.subjectoil degradersen
dc.titleThe potential of bacterial isolates for emulsification with a range of hydrocarbonsen
dc.typeArticleen
dc.contributor.departmentUniversity of Ulster. School of Biological/Environmental Sciences. Biotechnology Research Group.en
dc.identifier.journalActa Biotechnologicaen
ref.citationcount7 [Scopus, 13/05/2010]en
or.citation.harvardRahman, P. K. S. M. et. al. (2003) 'The potential of bacterial isolates for emulsification with a range of hydrocarbons', Acta Biotechnologica, 23 (4), pp.335-345.-
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