Novel technique for estimating cerebrovascular permeability demonstrates capsazepine protection following ischemia-reperfusion

Hdl Handle:
http://hdl.handle.net/10149/99155
Title:
Novel technique for estimating cerebrovascular permeability demonstrates capsazepine protection following ischemia-reperfusion
Authors:
Gauden, V. (Victoria); Hu, D-E. (De-En); Kurokawa, T. (Tsuyoshi); Sarker, M. H. (Mosharraf); Fraser, P. A. (Paul)
Affiliation:
King's College London. Cardiovascular Division.
Citation:
Gauden, V. et. al. (2007) 'Novel technique for estimating cerebrovascular permeability demonstrates capsazepine protection following ischemia-reperfusion', Microcirculation, 14 (8), pp.767-778.
Publisher:
Taylor & Francis
Journal:
Microcirculation
Issue Date:
Nov-2007
URI:
http://hdl.handle.net/10149/99155
DOI:
10.1080/10739680701410421
Abstract:
Objective: There has been some discussion as to whether the pial vasculature behaves in the same way as the blood-brain barrier as a whole. Recent studies have shown that capsazepine protects these vessels from the effects of ischemia-reperfusion. We have now used a new method to examine this protection in the whole brain. Methods: Horseradish peroxidase concentrations were measured in brain sections and plasma, following starch microsphere induced ischemia, which lasted from 20 to 60 minutes, with 30 minutes reperfusion. The PS product was calculated from the Crone-Renkin equation. Results: Permeability increase, which depended on duration of ischemia, was considerably greater in the pia than the parenchyma. The increase was also greater in tissue surrounding large radial venules of the cortex. Single vessel studies showed that these differences mirror those between small and large pial venules. Capsazepine treatment protected the parenchymal blood-brain barrier by limiting the post-ischemic permeability increase to about one third, but had no effect on the pia or radial vessel permeability. Conclusions: Permeability has been estimated in tissue sections with good spatial resolution using this new technique, which has demonstrated that the TRPV1 receptor plays an important role in the whole brain, not confined to small pial venules.
Type:
Article
Language:
en
Keywords:
blood-brain barrier; capillary permeability; capsazepine; ischemia reperfusion; TRPV1
ISSN:
1073-9688; 1549-8719
Rights:
Subject to restrictions, author can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 18/05/2010]
Citation Count:
2 [Scopus, 18/05/2010]

Full metadata record

DC FieldValue Language
dc.contributor.authorGauden, V. (Victoria)en
dc.contributor.authorHu, D-E. (De-En)en
dc.contributor.authorKurokawa, T. (Tsuyoshi)en
dc.contributor.authorSarker, M. H. (Mosharraf)en
dc.contributor.authorFraser, P. A. (Paul)en
dc.date.accessioned2010-05-18T15:28:02Z-
dc.date.available2010-05-18T15:28:02Z-
dc.date.issued2007-11-
dc.identifier.citationMicrocirculation; 14(8):767-778en
dc.identifier.issn1073-9688-
dc.identifier.issn1549-8719-
dc.identifier.doi10.1080/10739680701410421-
dc.identifier.urihttp://hdl.handle.net/10149/99155-
dc.description.abstractObjective: There has been some discussion as to whether the pial vasculature behaves in the same way as the blood-brain barrier as a whole. Recent studies have shown that capsazepine protects these vessels from the effects of ischemia-reperfusion. We have now used a new method to examine this protection in the whole brain. Methods: Horseradish peroxidase concentrations were measured in brain sections and plasma, following starch microsphere induced ischemia, which lasted from 20 to 60 minutes, with 30 minutes reperfusion. The PS product was calculated from the Crone-Renkin equation. Results: Permeability increase, which depended on duration of ischemia, was considerably greater in the pia than the parenchyma. The increase was also greater in tissue surrounding large radial venules of the cortex. Single vessel studies showed that these differences mirror those between small and large pial venules. Capsazepine treatment protected the parenchymal blood-brain barrier by limiting the post-ischemic permeability increase to about one third, but had no effect on the pia or radial vessel permeability. Conclusions: Permeability has been estimated in tissue sections with good spatial resolution using this new technique, which has demonstrated that the TRPV1 receptor plays an important role in the whole brain, not confined to small pial venules.en
dc.language.isoenen
dc.publisherTaylor & Francisen
dc.rightsSubject to restrictions, author can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 18/05/2010]en
dc.subjectblood-brain barrieren
dc.subjectcapillary permeabilityen
dc.subjectcapsazepineen
dc.subjectischemia reperfusionen
dc.subjectTRPV1en
dc.titleNovel technique for estimating cerebrovascular permeability demonstrates capsazepine protection following ischemia-reperfusionen
dc.typeArticleen
dc.contributor.departmentKing's College London. Cardiovascular Division.en
dc.identifier.journalMicrocirculationen
ref.citationcount2 [Scopus, 18/05/2010]en
or.citation.harvardGauden, V. et. al. (2007) 'Novel technique for estimating cerebrovascular permeability demonstrates capsazepine protection following ischemia-reperfusion', Microcirculation, 14 (8), pp.767-778.-
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