Microdiversity of phenol hydroxylase genes among phenol-degrading isolates of Alcaligenes sp. from an activated sludge system

Hdl Handle:
http://hdl.handle.net/10149/99234
Title:
Microdiversity of phenol hydroxylase genes among phenol-degrading isolates of Alcaligenes sp. from an activated sludge system
Authors:
Zhang, X. (Xueli); Gao, P. (Pingping); Chao, Q. (Qunfang); Wang, L. (Linghua); Senior, E. (Eric); Zhao, L. (Liping)
Affiliation:
Singapore Utilities International Pte Ltd. Centre for Advanced Water Technology. Innovation Centre (NTU).
Citation:
Zhang, X. et. al. (2004) 'Microdiversity of phenol hydroxylase genes among phenol-degrading isolates of Alcaligenes sp. from an activated sludge system', FEMS Microbiology Letters, 237 (2), pp.369-375.
Publisher:
Wiley-Blackwell
Journal:
FEMS Microbiology Letters
Issue Date:
2004
URI:
http://hdl.handle.net/10149/99234
DOI:
10.1111/j.1574-6968.2004.tb09719.x
Abstract:
Enterobacterial repetitive intergenic consensus (ERIC)-PCR fingerprinting classified 97 phenol-degrading isolates with identical amplified ribosomal DNA restriction analysis (ARDRA) patterns into six genotypic groups. The 16S rRNA gene of the representative isolate of each group had higher than 99.47% common identity with each other and higher than 98% identity with the type strain of Alcaligenes faecalis. PCR-TGGE (temperature gradient gel electrophoresis) analysis of the genes of the largest subunit of the multi-component phenol hydroxylase (LmPH) in each isolate followed with sequencing showed that isolates within each ERIC-PCR group had identical LmPH gene sequences. Among the six different ERIC-PCR groups, two were found to harbor two different LmPH genes encoding low- and high-Ks (affinity constants) phenol hydroxylases, and the low-Ks type LmPH was identical in sequence with one predominant LmPH of the parental activated sludge. Three ERIC-PCR groups had only the high-Ks type and one had no sequence similar to the known LmPHs. Our work suggests that there is no correlation between the phylogenetic groupings of phenol-degrading bacteria and their LmPH genotypes possibly due to extensive horizontal gene transfer of this functional gene.
Type:
Article
Language:
en
Keywords:
microdiversity; phenol hydoxylase; alcaligenes; horizontal gene transfer; activated sludge; ERIC-PCR fingerprinting
ISSN:
0378-1097; 1574-6968
Rights:
Subject to restrictions, author can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 19/05/2010]
Citation Count:
14 [Scopus, 19/05/2010]

Full metadata record

DC FieldValue Language
dc.contributor.authorZhang, X. (Xueli)en
dc.contributor.authorGao, P. (Pingping)en
dc.contributor.authorChao, Q. (Qunfang)en
dc.contributor.authorWang, L. (Linghua)en
dc.contributor.authorSenior, E. (Eric)en
dc.contributor.authorZhao, L. (Liping)en
dc.date.accessioned2010-05-19T08:53:21Z-
dc.date.available2010-05-19T08:53:21Z-
dc.date.issued2004-
dc.identifier.citationFEMS Microbiology Letters; 237(2):369-375en
dc.identifier.issn0378-1097-
dc.identifier.issn1574-6968-
dc.identifier.doi10.1111/j.1574-6968.2004.tb09719.x-
dc.identifier.urihttp://hdl.handle.net/10149/99234-
dc.description.abstractEnterobacterial repetitive intergenic consensus (ERIC)-PCR fingerprinting classified 97 phenol-degrading isolates with identical amplified ribosomal DNA restriction analysis (ARDRA) patterns into six genotypic groups. The 16S rRNA gene of the representative isolate of each group had higher than 99.47% common identity with each other and higher than 98% identity with the type strain of Alcaligenes faecalis. PCR-TGGE (temperature gradient gel electrophoresis) analysis of the genes of the largest subunit of the multi-component phenol hydroxylase (LmPH) in each isolate followed with sequencing showed that isolates within each ERIC-PCR group had identical LmPH gene sequences. Among the six different ERIC-PCR groups, two were found to harbor two different LmPH genes encoding low- and high-Ks (affinity constants) phenol hydroxylases, and the low-Ks type LmPH was identical in sequence with one predominant LmPH of the parental activated sludge. Three ERIC-PCR groups had only the high-Ks type and one had no sequence similar to the known LmPHs. Our work suggests that there is no correlation between the phylogenetic groupings of phenol-degrading bacteria and their LmPH genotypes possibly due to extensive horizontal gene transfer of this functional gene.en
dc.language.isoenen
dc.publisherWiley-Blackwellen
dc.rightsSubject to restrictions, author can archive post-print (ie final draft post-refereeing). For full details see http://www.sherpa.ac.uk/romeo/ [Accessed 19/05/2010]en
dc.subjectmicrodiversityen
dc.subjectphenol hydoxylaseen
dc.subjectalcaligenesen
dc.subjecthorizontal gene transferen
dc.subjectactivated sludgeen
dc.subjectERIC-PCR fingerprintingen
dc.titleMicrodiversity of phenol hydroxylase genes among phenol-degrading isolates of Alcaligenes sp. from an activated sludge systemen
dc.typeArticleen
dc.contributor.departmentSingapore Utilities International Pte Ltd. Centre for Advanced Water Technology. Innovation Centre (NTU).en
dc.identifier.journalFEMS Microbiology Lettersen
ref.citationcount14 [Scopus, 19/05/2010]en
or.citation.harvardZhang, X. et. al. (2004) 'Microdiversity of phenol hydroxylase genes among phenol-degrading isolates of Alcaligenes sp. from an activated sludge system', FEMS Microbiology Letters, 237 (2), pp.369-375.-
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